Chapter 13, Problem 3P

Now that the sequence of the entire E. coli K12 strain genome (roughly 5 Mb) is known, you can determine exactly where a cloned fragment of DNA came from in the genome by sequencing a few bases and matching that data with genomic information.

a.	About how many nucleotides of sequence information would you need to determine exactly where a fragment is from?
b.	If you had purified a protein from E. coli cells, roughly how many amino acids of that protein would you need to know to establish which gene encoded the protein?
c.	You determine 100 nucleotides of sequence of genomic DNA from a different E. coli strain, but you cannot find a match in the E. coli K12 genome sequence. How is this possible?