Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 11, Problem 5CHI
Summary Introduction
The microbial growth is considered to be an increase in the cell size or the number of microbial cells. The microbes exhibit binary fission, where the single cell divides into two cells. The growth of the microbes can be exhibited in growth curve that as four phases, such as
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Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution?
helpful formula:
g (generation time) = 0.301 (time)/ log x – log xo
bacterial suspension with a population of 6.3 x 107cells/mL was diluted as follows:
4 mL was removed and added to 16 mL of diluent. This was again diluted 1 in 300.
Determine the concentration of cells after EACH dilution.
Concentration after the 1st dilution
Concentration after the 2nd dilution
Below is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.
Chapter 11 Solutions
Prescott's Microbiology
Ch. 11.1 - Retrieve, Infer, Apply Discuss the ways in which...Ch. 11.1 - Describe the nutritional requirements of the major...Ch. 11.1 - Retrieve, Infer, Apply Compare...Ch. 11.1 - Retrieve, Infer, Apply What are the three major...Ch. 11.2 - Is NAD+ reduced to NADH in the catabolic or...Ch. 11.2 - Prob. 1RIACh. 11.2 - Why is it to a cells advantage to catabolize...Ch. 11.2 - Prob. 3RIACh. 11.4 - Which reactions are examples of substrate-level...Ch. 11.4 - For what kinds of reactions is NADPH used?
Ch. 11.4 - For what macromolecule is ribose 5-phosphate a...Ch. 11.4 - Summarize the major features of the...Ch. 11.4 - Prob. 2RIACh. 11.5 - Identify the substrate and products of the TCA...Ch. 11.5 - What chemical intermediate links pyruvate to the...Ch. 11.5 - Prob. 3RIACh. 11.5 - Retrieve, Infer, Apply In what eukaryotic...Ch. 11.5 - Why is it desirable for a microbe with the...Ch. 11.6 - Prob. 1MICh. 11.6 - Prob. 2MICh. 11.6 - Prob. 1RIACh. 11.6 - Describe the current model of oxidative...Ch. 11.6 - Prob. 3RIACh. 11.6 - Prob. 4RIACh. 11.7 - Prob. 1RIACh. 11.7 - Prob. 2RIACh. 11.7 - Prob. 3RIACh. 11.8 - Prob. 1MICh. 11.8 - Prob. 1RIACh. 11.8 - Prob. 2RIACh. 11.8 - Briefly describe alcoholic, lactic acid, mixed...Ch. 11.8 - Prob. 4RIACh. 11.8 - Prob. 5RIACh. 11.9 - What is the difference between a hydrolase and...Ch. 11.9 - Prob. 2MICh. 11.9 - Retrieve, Infer, Apply Briefly discuss the ways in...Ch. 11.9 - Prob. 2RIACh. 11.9 - Retrieve, Infer, Apply Describe how a...Ch. 11.10 - How do chemolithotrophs obtain their ATP and...Ch. 11.10 - Prob. 2RIACh. 11.10 - Why can hydrogen-oxidizing bacteria and archaea...Ch. 11.10 - What is reverse electron flow and why do many...Ch. 11.10 - Arsenate is a compound that inhibits...Ch. 11.11 - When electrons from P700 are used to reduce NADP+,...Ch. 11.11 - Define the following terms: light reactions, dark...Ch. 11.11 - Prob. 2RIACh. 11.11 - What is the function of accessory pigments?Ch. 11.11 - Prob. 4RIACh. 11.11 - Compare and contrast anoxygenic phototrophy and...Ch. 11.11 - Prob. 6RIACh. 11 - Without looking in chapters 21 and 22, predict...Ch. 11 - From an evolutionary perspective, discuss why most...Ch. 11 - How would you isolate a thermophilic...Ch. 11 - Certain chemicals block ATP synthesis by allowing...Ch. 11 - Prob. 5CHICh. 11 - A cyanobacterium having photosystem I but not...Ch. 11 - Review the description of the Berkeley Pit Lake in...Ch. 11 - The archaeon Metallosphaera sedula is of great...Ch. 11 - Nitrite-oxidizing bacteria have been thought to be...
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- If the sterile glycerol stock is 40%, what will be the volume of the bacterial culture in a nutrient broth that must be added to have a final concentration necessary for a glycerol stock culture? the final volume in the microfuge tube must be 1ml. Compute. Answers should be in a microliter.arrow_forwardThis is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to answer the following questions. 3 Cell concentration (million cells/ml) 2.5 2 0.2 1.5 1 0.5 0 2 The growth rate is lag log 0.25 4 0.32 0.5 units 6 million of cells per ml (Image description.: Horizontal axis is time in hours, Vertical is cell concentration in millions of cells per ml. Curve starts at 0,0, increases by 4 hours to 0.5 million cells/ml, goes up steeply over two hours from 0.5 million/ml at 4 hours to 2.5 million cells/ml at 8 hours, 8-12 hour shows an increase of 0.3 to 2,8 million cellls/ml and last 2 hours is flat at 2.8million cells/ml.) From 12 hr to 14 hr is called phase. lunar 2 8 Time (hours) 10 5 stationary 12 cells 14 death 16 million of cells per ml per hour hours million of cellsarrow_forwardThe number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution -3 Plate 1 10 dilution Plate 2 10-4 dilution Plate 3 10-5 dilution Plate 4 10 dilution Plate 5 10 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 1,957 65 21 1 0 marrow_forward
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