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You mutagenized bacteria and isolated two mutants that you suspected to be defective in DNA synthesis. You measured incorporation of radioactivity (Y- axis) for various times (X-axis) at two temperatures (30 deg C shown as open circles and 40 deg C shown a filled circles in both figures) for the two mutants of bacteria (left and right figures). For this experiment, you can conclude that 20,000 1,000- 30° 0- 15,000- FAZ 10,000- 500 5,000- 0 40 80 0 40 80 120 120 Time (min) None of these conclusions You have isolated a component of RNA synthesis that is temperature insensitive O You have isolated mutants that are temperature insensitive O You have isolated temperature-sensitive mutants that are defective in DNA synthesis ³H-thymidine incorporated (cpm) 40°

You mutagenized bacteria and isolated two mutants that you suspected to be defective in DNA synthesis. You measured incorporation of radioactivity (Y- axis) for various times (X-axis) at two temperatures (30 deg C shown as open circles and 40 deg C shown a filled circles in both figures) for the two mutants of bacteria (left and right figures). For this experiment, you can conclude that 20,000 1,000- 30° 0- 15,000- FAZ 10,000- 500 5,000- 0 40 80 0 40 80 120 120 Time (min) None of these conclusions You have isolated a component of RNA synthesis that is temperature insensitive O You have isolated mutants that are temperature insensitive O You have isolated temperature-sensitive mutants that are defective in DNA synthesis ³H-thymidine incorporated (cpm) 40°

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Experiment Summary** You mutagenized bacteria and isolated two mutants that you suspected to be defective in DNA synthesis. You measured the incorporation of radioactivity (Y-axis) for various times (X-axis) at two temperatures (30°C shown as open circles and 40°C shown as filled circles in both figures) for the two mutants of bacteria (left and right figures). For this experiment, you can conclude that: **Figures Explanation:** - **Left Graph:** - **X-axis:** Time (min) - **Y-axis:** ³H-thymidine incorporated (cpm) - **Data Points:** - Open circles represent data at 30°C - Filled circles represent data at 40°C - **Right Graph:** - **X-axis:** Time (min) - **Y-axis:** ³H-thymidine incorporated (cpm) - **Data Points:** - Open circles represent data at 30°C - Filled circles represent data at 40°C In both graphs: - At 30°C (open circles), there is a significant increase in the incorporation of ³H-thymidine over time. - At 40°C (filled circles), the incorporation of ³H-thymidine does not rise and remains minimal throughout the experiment duration. Given the data, select the most appropriate conclusion from the options provided: 1. None of these conclusions 2. You have isolated a component of RNA synthesis that is temperature insensitive 3. You have isolated mutants that are temperature insensitive 4. You have isolated temperature-sensitive mutants that are defective in DNA synthesis 5. You have isolated a component of protein synthesis The data supports conclusion (4), as the mutants demonstrate a significant defect in DNA synthesis at the higher temperature (40°C) while functioning normally at the lower temperature (30°C), indicating that they are temperature-sensitive mutants.
Transcribed Image Text:**Experiment Summary** You mutagenized bacteria and isolated two mutants that you suspected to be defective in DNA synthesis. You measured the incorporation of radioactivity (Y-axis) for various times (X-axis) at two temperatures (30°C shown as open circles and 40°C shown as filled circles in both figures) for the two mutants of bacteria (left and right figures). For this experiment, you can conclude that: **Figures Explanation:** - **Left Graph:** - **X-axis:** Time (min) - **Y-axis:** ³H-thymidine incorporated (cpm) - **Data Points:** - Open circles represent data at 30°C - Filled circles represent data at 40°C - **Right Graph:** - **X-axis:** Time (min) - **Y-axis:** ³H-thymidine incorporated (cpm) - **Data Points:** - Open circles represent data at 30°C - Filled circles represent data at 40°C In both graphs: - At 30°C (open circles), there is a significant increase in the incorporation of ³H-thymidine over time. - At 40°C (filled circles), the incorporation of ³H-thymidine does not rise and remains minimal throughout the experiment duration. Given the data, select the most appropriate conclusion from the options provided: 1. None of these conclusions 2. You have isolated a component of RNA synthesis that is temperature insensitive 3. You have isolated mutants that are temperature insensitive 4. You have isolated temperature-sensitive mutants that are defective in DNA synthesis 5. You have isolated a component of protein synthesis The data supports conclusion (4), as the mutants demonstrate a significant defect in DNA synthesis at the higher temperature (40°C) while functioning normally at the lower temperature (30°C), indicating that they are temperature-sensitive mutants.
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