A geneticist isolates two mutations in a bacteriophage. One mutation causes clear plaques (c), and the other produces minute plaques (m). Previous mapping experiments have established that the genes responsible for these two mutations are 8 m.u. apart. The geneticist mixes phages with genotype c+ m+ and genotype c− m− and uses the mixture to infect bacterial cells. She collects the progeny phages and cultures a sample of them on plated bacteria. A total of 1000 plaques are observed. What numbers of the different types of plaques (c+ m+, c− m−, c+ m−, c− m+) should she expect to see?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A geneticist isolates two mutations in a bacteriophage. One
mutation causes clear plaques (c), and the other produces minute
plaques (m). Previous mapping experiments have established that
the genes responsible for these two mutations are 8 m.u. apart. The
geneticist mixes phages with genotype c+ m+ and genotype c− m−
and uses the mixture to infect bacterial cells. She collects the
progeny phages and cultures a sample of them on plated bacteria. A
total of 1000 plaques are observed. What numbers of the different
types of plaques (c+ m+, c− m−, c+ m−, c− m+) should she expect to
see?
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