You have been given 5 protein samples of known concentrations (ug ml) for SDS-PAGE analysis: a. Wild Type 1: 900 ug ml b. Wild Type 2: 500 ug ml C. Arr Mutant: 750 ug ml d. Dyn Mutant: 450 ug ml e. GRK Mutant: 800 ug ml You are required to load the same total protein mass into each well, which can hold up to 25 ul of a protein sample (don't worry about accounting for loadin sample is needed to accomplish this? Please use a new line for each sample preceded by the corresponding letter. Don't forget units, as shown in the following example: a. Arr: 10 ml b. WT1: 5g, (25 ml) c. GRK: 20 ml
Structure and Composition of Cell Membrane
Despite differences in structure and function, all living cells in multicellular organisms are surrounded by a cell membrane. Just like the outer layer of the skin separates the body from its environment similarly, the cell membrane, also known as 'plasma membrane,' separates the inner content from its exterior environment.
Cell Membrane
The cell membrane is known by different names like plasma membrane or cytoplasmic membrane, or biological membrane. The term "cell membrane" was first introduced by C. Nageli and C. Cramer in the year 1855. Later on, in 1931, the term "plasmalemma" for cell membrane was given by J. Plowe. The cell membrane separates the cell's internal environment from the extracellular space. This separation allows the protection of cells from their environment.
Prokaryotes vs Eukaryotes
The cell is defined as the basic structural and functional unit of life. The cell membrane bounds it. It is capable of independent existence.
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