You are given an unknown bacterial culture and you want to determine if it is resistant to antibiotics To test this, you plate the bacterial culture on two plates, one that has streptomycin (an antibiotic) and one without streptomycin. Which of the following outcomes would you most likely observe if the bacteria is antibiotic resistant? No bacterial growth on either plate O Bacterial growth on both plates Bacterial growth on only the plate with streptomycin Bacterial growth on only the plate without streptomycin

You are given an unknown bacterial culture and you want to determine if it is resistant to antibiotics To test this, you plate the bacterial culture on two plates, one that has streptomycin (an antibiotic) and one without streptomycin. Which of the following outcomes would you most likely observe if the bacteria is antibiotic resistant? No bacterial growth on either plate O Bacterial growth on both plates Bacterial growth on only the plate with streptomycin Bacterial growth on only the plate without streptomycin

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 1CR

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When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to spread the bacteria evenly across the plate before you add the antibiotic. Why do the bacteria need to be applied evenly? None of the following are true All of the following are true If you add more bacteria in some areas compared to others, it might obscure the effect of the antibiotic If the area right next to the antibiotic initially received relatively few bacterial cells, that might lead you to overestimate the effectiveness of the antibiotic If the area right next to the antibiotic initially received a relatively large number of bacterial cells, that might lead you to underestimate the effectiveness of the antibiotic
Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?
Would you use an inoculating loop to transfer bacteria to an agar deep tube? Would you use an inoculating loop to transfer bacteria to an agar deep tube? Yes, as long as you insert the loop into the agar smoothly. No, a needle would be best since it will do the least amount of damage to the agar and allow it to reseal around the inserted bacteria. It depends on which bacteria you are transferring. Yes, as long as you are careful when streaking on the surface of the agar.
A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?
You have a mixed culture of 2 different bacterial species. One of the species is Gram negative bacilli and the other species is Gram positive cocci. You have noticed that one species is killed by penicillin antibiotics (attacks peptidoglycan) while the other survives. Neither species has antibiotic resistance genes. How can you explain this result? Remember to make direct reference to each bacterial species in your response
When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times with the loop?
You were tasked to prepare four petri plates. Three of the petri dishes were inoculated using different samples. The plates were prepared from the following: Plate A: oral swab after brushing Plate B: cat’s paw Plate C: exposed in air for one hour Plate D: uninoculated The plates were incubated at 37˚C for 24 hours. The following results were obtained: 1. Compare the growth of the cultures found in Plates A, B, C and D. Which among the four plates has shown the most growth? Why do you think so? 2. Describe the appearance and abundance of the colonies found in your four plates. What possible type of microorganisms are found in your plates? 3. Although sparse, there is still visible growth of microorganisms in Plate D (uninoculated plate). Where do you think the microorganism came from? REFERENCES:
You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flask
Using your fingers, you are asked to aseptically touch the surface of a sterile agar plate. Illustrate the possible result from this step if your fingers are (a) unwashed – touched various things prior to placing on agar surface, and (b) washed with soap or disinfected with 70% alcohol. Describe the relative abundance of microbial growth observed on the plates. List and draw the possible characteristics of an isolated bacterial colony that can be observed based on type of (a) margin, (b) elevation, (c) texture, and (d) optical property.
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?
With your tests you now figured out that your patient has a Staphylococcus infection, and you would like to know if the infection is caused by Staphylococcus aureus or by Staphylococcus epidermis. What test can you do next to see if your patient indeed has a Staphylococcus aureus infection? O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus aureus can ferment mannitol turning the agar plate yellow. Staphylococcus erpidermis cannot ferment mannitol and the agar plate stays red. O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus epidermis can ferment mannitol turning the agar plate yellow. Staphylococcus aureus cannot ferment mannitol and the agar plate stays red. O There is no way you can distinguish between Staphylococcus aureus and Staphylococcus erpidermis O you do a Gram stain. Staphylococcus aureus will stay purple as it is Gram positive and Staphylococcus erpidermis will show up as…