What would be the expected out come of a gram staining test If the bacteria is a pathogenic intestinal bacteria?

Q: There are two bacteria, and I have to find out the organism. I did gram staining, and I found out…

A: Gram staining is a laboratory technique used to differentiate and classify bacteria into two major…

Q: Three grams of soil from the ground were added to 27 mL of sterile water and shaken vigorously.…

A: The initial dilution was a 1/100 dilution, followed by 4 1/10 dilutions. So, the calculate the CFU…

Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…

A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…

Q: A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL…

A: It is given that a culture of Staphylococcus is diluted as follows:1. The original 20mL of culture…

Q: Kousei works at a company that produces sweetened condensed milk. If one or more samples exceed 10…

A: Cell count: The cell count data is generally utilized to predict the number of living microbes…

Q: You are hired in one of the microbiology labs in Memphis and your first assignment to identify…

A: The last reagent used in Gram staining, which is crucial for differentiating between Gram-positive…

Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?

A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: The question asks about the test that can be used on the disease-causing organism below that can…

Q: A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine…

A: To keep the number of colonies on the plate within a certain range, serial dilutions of culture are…

Q: Is bile esculin test a efficient test for unknown intestinal bacteria?

A: In the intestine, only those bacteria that are not killed by the bile survive. The normal bacteria…

Q: A pure culture was inoculated onto a Mueller-Hinton agar plate. The Kirby-Bauer procedure was…

A: The Kirby-Bauer procedure also known as a disk diffusion test is performed to test the antibiotic…

Q: How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect?…

A: Several tests are performed in the laboratory in order to detect several bacterial agents as well as…

Q: Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.

A: The Sabin Feldman dye test is used to diagnose the toxoplasmosis, which is a disease caused by…

Q: To determine the number of cells in a pure culture of Klebsiella pneumoniae, you have performed a…

A: Colony-forming units per millilitre (cfu/ml) of Klebsiella pneumonia original culture can be found…

Q: You mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do…

A: Differential media is a type of growth media used in the labs to grow and differentiate or…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: A dichotomous key is a tool made by the scientist to identify any living being based on its…

Q: The student advises using the same selection procedures and inoculating a 5 mL overnight culture…

A: Quantification: The process of plating on agar plates enables the counting of individual colonies,…

Q: You want to isolate staphylococci from a clinical sample, as you suspect the patient nas an…

A: Staphylococcus aureus : It is gram positive , round shaped bacteria and is a usual member of…

Q: You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions…

A: Hi, since there are multiple questions posted we will provide you with answers for the first…

Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…

A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…

Q: during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri…

A: Introduction :- Inoculation is the process of intentionally spreading a mild strain of the illness,…

Q: Out of twelve tests that can be performed:  Gram Stain Wet Mount Endospore Staining Acid Fast…

A: The unknown bacteria can be identified by morphological and biochemical methods. The method of…

Q: In the image attached is a unknown bacteria lab. The gram stain result and growth result is already…

A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…

Q: Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies…

A: Microorganisms are small organism that cannot be seen by naked eyes. Microorganism can be harmful to…

Q: You have performed an experiment and your aim is to obtain pure colonies. However, you observe some…

A: Bacteria are prokaryotic organisms. They are microscopic single-celled organisms that can live in a…

Q: You perform a Gram stain of this mixed culture as a control while simultaneously Gram staini an…

A: In order to answer this question we need to know the exact Gram staining procedure which is as…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.