Why might it be necessary to include the 50 mL cultures in order to express protein? This question is referring to a lab experiment for protein expression. We streaked our plates. Put 50uL of two antibodies (AMP and CAM) into 50mL LB broth. Then took a colony and mixed it in the flask with LB broth and antibodies. The next step is to incubate the flasks overnight. Then add 500uL of AMP to 500mL of LB broth. We will add 25mL of the overnight culture to the 500mL LB broth. Hopefully this is enough detail to answer the question.
Why might it be necessary to include the 50 mL cultures in order to express protein? This question is referring to a lab experiment for protein expression. We streaked our plates. Put 50uL of two antibodies (AMP and CAM) into 50mL LB broth. Then took a colony and mixed it in the flask with LB broth and antibodies. The next step is to incubate the flasks overnight. Then add 500uL of AMP to 500mL of LB broth. We will add 25mL of the overnight culture to the 500mL LB broth. Hopefully this is enough detail to answer the question.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Why might it be necessary to include the 50 mL cultures in order to express protein?
This question is referring to a lab experiment for protein expression. We streaked our plates. Put 50uL of two antibodies (AMP and CAM) into 50mL LB broth. Then took a colony and mixed it in the flask with LB broth and antibodies. The next step is to incubate the flasks overnight. Then add 500uL of AMP to 500mL of LB broth. We will add 25mL of the overnight culture to the 500mL LB broth. Hopefully this is enough detail to answer the question. Thank you!..
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON