Human Immunodeficiency Virus (HIV) Principle: The assay starts with a sample applied to the sample well. A recombinant HIV antigen conjugated to colloidal gold embedded in the sample pad reacts with the HIV antibody present in serum or plasma forming conjugate / HIV antibody complex. As the mixture is allowed to migrate along the test strip, the conjugate / HIV antibody complex is captured by recombinant HIV antigen immobilized on a membrane forming a colored test band in the test region. A negative sample does not produce a test band due to the absence of colloidal gold conjugate / HIV antibody complex. The antigens used in the conjugate test are recombinant proteins that correspond to highly immunoreactive regions of HIV 1 and HIV 2. A colored control band in the control region appears at the end of test procedure regardless of test result. This control band is the result of colloidal gold conjugate binding to the anti-HIV antibody immobilized on the membrane. The control band indicates that the colloidal gold conjugate is functional. Procedure: DO NOT OPEN POUCH UNTIL YOU ARE READY TO TEST THE SAMPLE. For test cards: 1. Bring all reagents and specimens to room temperature. 2. Remove the test card from the foil pouch and place on a clean dry surface. 3. Identify the test card for each specimen or control. 4. Dispense 100 ul (3 drops) of the specimen or control into the sample well on the card. 5. Interpret test results at 15 minutes. Caution: Use a clean pipette or tip for every sample to avoid cross – contamination. Note: A positive result may be interpreted early, however read any negative at 15 minutes to ensure sample is negative and not a low concentration of the anti-HIV antibody. DO NOT INTERPRET THE RESULT AFTER 20 MINUTES. Questions 1. What is HIV? 2. Discuss what an immunochromatographic assay is 3. If a positive band appears in the test region but there is no band in the control region, will you accept the result

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Human Immunodeficiency Virus (HIV) Principle: The assay starts with a sample applied to the sample well. A recombinant HIV antigen conjugated to colloidal gold embedded in the sample pad reacts with the HIV antibody present in serum or plasma forming conjugate / HIV antibody complex. As the mixture is allowed to migrate along the test strip, the conjugate / HIV antibody complex is captured by recombinant HIV antigen immobilized on a membrane forming a colored test band in the test region. A negative sample does not produce a test band due to the absence of colloidal gold conjugate / HIV antibody complex. The antigens used in the conjugate test are recombinant proteins that correspond to highly immunoreactive regions of HIV 1 and HIV 2. A colored control band in the control region appears at the end of test procedure regardless of test result. This control band is the result of colloidal gold conjugate binding to the anti-HIV antibody immobilized on the membrane. The control band indicates that the colloidal gold conjugate is functional. Procedure: DO NOT OPEN POUCH UNTIL YOU ARE READY TO TEST THE SAMPLE. For test cards: 1. Bring all reagents and specimens to room temperature. 2. Remove the test card from the foil pouch and place on a clean dry surface. 3. Identify the test card for each specimen or control. 4. Dispense 100 ul (3 drops) of the specimen or control into the sample well on the card. 5. Interpret test results at 15 minutes. Caution: Use a clean pipette or tip for every sample to avoid cross – contamination. Note: A positive result may be interpreted early, however read any negative at 15 minutes to ensure sample is negative and not a low concentration of the anti-HIV antibody. DO NOT INTERPRET THE RESULT AFTER 20 MINUTES. Questions 1. What is HIV? 2. Discuss what an immunochromatographic assay is 3. If a positive band appears in the test region but there is no band in the control region, will you accept the result?

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