ammonium chloride on the infection of CER cells'

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The early steps of rabiesvirus (CVS) infection in vitro were studied in chicken embryo cells
(CER) cells. The infection was monitored by looking for specific viral inclusions using anti-rabies
fluorescein isothiocynanate at 24 hours after addition of virus. Cells were incubated for various
lengths of time after virus attachment with ammonium chloride and chloroquine. The results are
show in Figure 2.
100
75
50
25
1 3 5
7.
24
Time (h)
(1) 20 mM NHẠCI
(0) 0.1 mM chloroquine
Figure 2
What is the effect of ammonium chloride on the infection of CER cells? Explain why
there are such effects.
Fluorescence inhibition (%)
Transcribed Image Text:The early steps of rabiesvirus (CVS) infection in vitro were studied in chicken embryo cells (CER) cells. The infection was monitored by looking for specific viral inclusions using anti-rabies fluorescein isothiocynanate at 24 hours after addition of virus. Cells were incubated for various lengths of time after virus attachment with ammonium chloride and chloroquine. The results are show in Figure 2. 100 75 50 25 1 3 5 7. 24 Time (h) (1) 20 mM NHẠCI (0) 0.1 mM chloroquine Figure 2 What is the effect of ammonium chloride on the infection of CER cells? Explain why there are such effects. Fluorescence inhibition (%)
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