During the incubation step of the plaque assay, viral replication leads to host cell lysis. This allows the freshly-released virions to diffuse through the media and infect new hosts. The amount of agar added to the top agar is 7 g per liter rather than the 16 g of agar per liter for most solid media, including the bottom agar used in this assay. How would it change the plaque assay results if bottom agar was used in place of the top agar in this assay? a- The diameter of the plaques would be smaller. b- The diameter of the plaques would not change. c- The diameter of the plaques would be larger. (2) A researcher measured the viral titer of PhiX174 in fish tank water, untreated sewage water, and unpasteurized apple juice. Which sample do you predict will have the highest titer on this page? a- Fish tank water b- Unpasteurized apple juice c- Untreated sewage water
During the incubation step of the plaque assay, viral replication leads to host cell lysis. This allows the freshly-released virions to diffuse through the media and infect new hosts. The amount of agar added to the top agar is 7 g per liter rather than the 16 g of agar per liter for most solid media, including the bottom agar used in this assay. How would it change the plaque assay results if bottom agar was used in place of the top agar in this assay? a- The diameter of the plaques would be smaller. b- The diameter of the plaques would not change. c- The diameter of the plaques would be larger. (2) A researcher measured the viral titer of PhiX174 in fish tank water, untreated sewage water, and unpasteurized apple juice. Which sample do you predict will have the highest titer on this page? a- Fish tank water b- Unpasteurized apple juice c- Untreated sewage water
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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During the incubation step of the plaque assay, viral replication leads to host cell lysis. This allows the freshly-released virions to diffuse through the media and infect new hosts. The amount of agar added to the top agar is 7 g per liter rather than the 16 g of agar per liter for most solid media, including the bottom agar used in this assay. How would it change the plaque assay results if bottom agar was used in place of the top agar in this assay?
a- The diameter of the plaques would be smaller.
b- The diameter of the plaques would not change.
c- The diameter of the plaques would be larger.
(2) A researcher measured the viral titer of PhiX174 in fish tank water, untreated sewage water, and unpasteurized apple juice. Which sample do you predict will have the highest titer on this page?
a- Fish tank water
b- Unpasteurized apple juice
c- Untreated sewage water
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