Ms. Carla was required to clone three different genes (Gcne 1, Gene 2 and Gene 3) intoPUC18. After generating three recombinant plasmids, she transformed them into theEscherichiu coli host cells and plated them on the LB agar plates containing X-gal. After a16-hour incubation peried, she obtained different outcomes for all of her three genes ofinterest.Gene 1 plate: No colonies ut allGene 2 plate: All blue coloniesGene 3 plate: All white coloniesExplain the results obtained for cach plate with clear justification.

Blue-white screening technique that can be used to identify the recombinant bacteria which have…

Answered: Ms. Carla was required to clone three…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

can you explain why Bacillus anthracis can be pathogenic in a mouse and not be fought off by the immune system? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
Why are there strict reminders about mixing in the inverted position and not vortexing Plasmid samples? Will these actions affect the plamid DNA extraction?
The plaque assay plate below was made from a dilution of 10-7 and 0.1 ml of the dilution was plated on the cell monolayer. What is the virus titer in PFU/ml? Note: 10e1 means 10 to the first power, etc. Select all answers that apply.
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The cells were incubated for 48 hours after transfection prior to harvesting. The reason for waiting this amount time is: the Transfection takes that long to occur. It allows the cell to transcribe and translate the transfected DNA
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In figure 3, what do the letters C, S, P, and m indicate in panel A? I need help finding the answer in the article link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
Have a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a sample of recombinant protein "LBT3" was loaded. Lane A shows proteins that have been prepared from leukocytes from a normal, healthy individual. Lanes B, C, D, E and F show protein prepared from leukocytes from individuals with lymphoma. What is the best explanation for the result shown for patient D? 200 150 100 75 50 37 20 Weight, kDa MW ladder Control A
In our pandemic world, COVID testing has become a very common thing (unfortunately necessary). When receiving a test to detect the COVID virus, a person may or may not have COVID, and the test result could come back either positive or negative. (a) Explain what specificity means in the context of a COVID test. (b) Explain what sensitivity means in the context of a COVID test.
A student has forgotten which antibiotic plasmid she used in her E. coli transformation. It could have been kanamycin, ampicillin, or tetracycline. She decides to make up a special set of plates to determine the type of antibiotic used. The plates below show the results of the test. Plate IV. LB agar Plate I. LB agar with kanamycin Plate II. LB agar with ampicillin Plate III. LB agar with tetracycline 13. Which antibiotic plasmid has been used? a. kanamycin c. tetracycline b. ampicillin 14. What is the explanation for these results? a. Plates I and Il each contain a plasmid that is resistant to that antibiotic. b. Plate III has antibiotic agar, but E. coli that has been transformed to be resistant to tetracycline can grow. c. Plate IV has no antibiotic. d. There are no tetracycline-resistant cells on Plate II.

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