A student has forgotten which antibiotic plasmid she used in her E. coli transformation. It could have been kanamycin, ampicillin, or tetracycline. She decides to make up a special set of plates to determine the type of antibiotic used. The plates below show the results of the test. Plate IV. LB agar Plate I. LB agar with kanamycin Plate II. LB agar with ampicillin Plate III. LB agar with tetracycline 13. Which antibiotic plasmid has been used? a. kanamycin c. tetracycline b. ampicillin 14. What is the explanation for these results? a. Plates I and Il each contain a plasmid that is resistant to that antibiotic. b. Plate III has antibiotic agar, but E. coli that has been transformed to be resistant to tetracycline can grow. c. Plate IV has no antibiotic. d. There are no tetracycline-resistant cells on Plate II.
A student has forgotten which antibiotic plasmid she used in her E. coli transformation. It could have been kanamycin, ampicillin, or tetracycline. She decides to make up a special set of plates to determine the type of antibiotic used. The plates below show the results of the test. Plate IV. LB agar Plate I. LB agar with kanamycin Plate II. LB agar with ampicillin Plate III. LB agar with tetracycline 13. Which antibiotic plasmid has been used? a. kanamycin c. tetracycline b. ampicillin 14. What is the explanation for these results? a. Plates I and Il each contain a plasmid that is resistant to that antibiotic. b. Plate III has antibiotic agar, but E. coli that has been transformed to be resistant to tetracycline can grow. c. Plate IV has no antibiotic. d. There are no tetracycline-resistant cells on Plate II.
Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 25RQ
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Transcribed Image Text:A student has forgotten which antibiotic plasmid she used in her E. coli transformation. It could have been
kanamycin, ampicillin, or tetracycline. She decides to make up a special set of plates to determine the type of
antibiotic used. The plates below show the results of the test.
Plate IV. LB agar
Plate I. LB agar
with kanamycin
Plate II. LB agar
with ampicillin
Plate III. LB agar
with tetracycline
13. Which antibiotic plasmid has been used?
a. kanamycin
c. tetracycline
b. ampicillin
14. What is the explanation for these results?
a. Plates I and Il each contain a plasmid that is resistant to that antibiotic.
b. Plate III has antibiotic agar, but E. coli that has been transformed to be resistant to tetracycline can grow.
c. Plate IV has no antibiotic.
d. There are no tetracycline-resistant cells on Plate II.
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