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Why is there an inverse relationship between the dilution factor and the amount of virus in solution?
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- Describe three problems associated with using the standard plate count method for determining the number of bacteria in a sample.why In spread-plate technique, the bacterial suspension volume should not exceed 0.1ml.You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml. a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution b) What is the dilution factor in each step, in the same order in which the dilutions are made? c) What is the total serial dilution factor?
- 1) What are all of the different colony types that can be seen on a mixed-culture streak plate? 2) What kinds of colonies can be seen when mixed cultures of E. coli, M. luteus, and Serratia marcesens? 3) What is the range of colonies that may be seen with each mixed culture?1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original concentration in the sample? b) If you were to dilute the original sample with a dilution factor of 108, how many colonies would you count on the plate? c) If you were to dilute the original sample with a dilution factor of 106, how many colonies would you count on the plate? 2. Sally Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a serial dilution of a culture of Yersinia pestis. She had pipetted 1 ml sample of diluted Yersinia pestis from a tube with the overall dilution factor of 106 into a plate and covered it with agar. She used this plate to calculate the concentration in cells/ml of a bacterial culture. a) How many organisms were in the original culture? b) Were her results valid? Why or why not?Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.
- You are testing a river water sample. Your "original" plate shows 100 coliform colonies. Assuming you did the serial dilution correctly, how many coliform colonies would you expect to see on your 1:10 plate? Your 1:100 plate? Explain.What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?Why is that bacterial slide agglutination technique important in diagnostic procedure?
- What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name the factors that can compromise the effectiveness of a disinfecting agentThe volume of E. coli added to each warm agar pour/virus mixture was originally100µL. After pre-testing the experiment, the instructions were modified for you to add 300µL of E. coli. Make an educated guess as to why the volume was increased.Please describe the pros and cons of the following methods for counting bacterial cells. 1) Petroff-Hausser counting chamber 2) Coulter Counter 3) Plate Count