There are two figures- one for vector control plate & another PCRinsert+vector experimental plate.  Control plate = 10 blue colonies ,  Ligation plate ( experimental) = 150 white , 8 blue. Interpret what each figure means, what is its importance (control and experimental)? Why do colonies grow on vector control plates? Why are both blue and white colonies in your experimental plate? What were the components of the agar plate & their use (AMP+Xgal+IPTG)? What do these suggest about amp sensitivity and resistance? Why did we use X-gal and IPTG? Can you think of any issue with your result? Is the recombination efficiency good? Is your transformation efficiency good?  •Write a concluding sentence about this lab and the data you analyzed.

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.