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What is the correct order to prepare pure recombinant proteins when using E coli expression system? 1. Select appropriate column chromatography to purify the recombinant proteins

What is the correct order to prepare pure recombinant proteins when using E coli expression system? 1. Select appropriate column chromatography to purify the recombinant proteins

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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What is the correct order to prepare pure recombinant proteins when using E coli expression system? 1. Select appropriate column chromatography to purify the recombinant proteins 2. Make a DNA plasmid containing gene that codes target protein 3. Transform the DNA plasmids into E. coli cells 3. Grow E. coli cells in appropriate media for overexpressing recombinant proteins by IPTG and harvest cells 4. Break open the cells to release the recombinant proteins into appropriate buffer and spin down to get supernatant part
Transcribed Image Text:What is the correct order to prepare pure recombinant proteins when using E coli expression system? 1. Select appropriate column chromatography to purify the recombinant proteins 2. Make a DNA plasmid containing gene that codes target protein 3. Transform the DNA plasmids into E. coli cells 3. Grow E. coli cells in appropriate media for overexpressing recombinant proteins by IPTG and harvest cells 4. Break open the cells to release the recombinant proteins into appropriate buffer and spin down to get supernatant part
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