The text has discussed the RNA-Seq technique, whichquantifies the amounts of mRNAs present in a given celltype at a given time. We have also described the methodof ribosome profiling, which quantifies the levels ofmRNA sequences that are being translated actively in acell population at a given time. But even when bothkinds of experiments are performed, the data do not tellus accurately the relative amounts of the protein products of these mRNAs in the cells at that time. Why not?What elements of gene expression do these techniquesaccount for and what elements do they ignore?
The text has discussed the RNA-Seq technique, whichquantifies the amounts of mRNAs present in a given celltype at a given time. We have also described the methodof ribosome profiling, which quantifies the levels ofmRNA sequences that are being translated actively in acell population at a given time. But even when bothkinds of experiments are performed, the data do not tellus accurately the relative amounts of the protein products of these mRNAs in the cells at that time. Why not?What elements of gene expression do these techniquesaccount for and what elements do they ignore?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The text has discussed the RNA-Seq technique, which
quantifies the amounts of mRNAs present in a given cell
type at a given time. We have also described the method
of ribosome profiling, which quantifies the levels of
mRNA sequences that are being translated actively in a
cell population at a given time. But even when both
kinds of experiments are performed, the data do not tell
us accurately the relative amounts of the protein products of these mRNAs in the cells at that time. Why not?
What elements of gene expression do these techniques
account for and what elements do they ignore?
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