. The mechanism for lysozyme cleavage of its polysaccharide substrate requires Glu35 in itsnonionized form, whereas the nearby Asp52 must be ionized (see the figure below). The pKvalues for the side-chain carboxyl groups on the two amino acids in solution are virtuallyidentical.a) How can one carboxyl group be charged and the other uncharged in the active site oflysozyme?b) The pH optimum for lysozyme is about 5. Why do you suppose that the activity decreasesabove and below this optimum?Glu3sNAGAsp520-HGlu35 -CAsp52tri-NAGNAG

The lysozyme is an enzyme found in the secretions like tears and sweat. It cleaves the glycosidic…

Answered: The mechanism for lysozyme cleavage of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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