Bacterial species that are capable of synthesising the amino acid histidine do not need it in their growth medium. Histidine biosynthesis requires a specific enzyme, which we shall refer to as histidine synthetase. When these bacteria are supplemented

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Bacterial species that are capable of synthesising the amino acid histidine do not need it in their growth medium. Histidine biosynthesis requires a specific enzyme, which we shall refer to as histidine synthetase. When these bacteria are supplemented with histidine in their growth media, they cease intracellular histidine synthesis. Propose three distinct regulatory mechanisms to account for why histidine production ends when histidine is present in the growth medium. To further investigate this phenomena, you analyse the quantity of intracellular histidine synthetase protein produced when cells are cultured with and without histidine. The quantity of this protein is same in both scenarios. Which regulatory mechanism makes sense in light of this observation?

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