A Cys residue is required for the transport function of a bacterial lactose transporter that is highly selective for lactose. The transporter is permanently inactivated by the covalent interaction of N-ethylmaleimide (NEM) with this Cys residue. A high lactose content in the medium protects the Cys residue at or near the lactose-binding site from inactivation by NEM, possibly by sterically protecting it. You are unaware of any more information regarding the transporter protein. Suggestions for experiments that might be used to determine the Mr of this Cys-containing transporter polypeptide
A Cys residue is required for the transport function of a bacterial lactose transporter that is highly selective for lactose. The transporter is permanently inactivated by the covalent interaction of N-ethylmaleimide (NEM) with this Cys residue. A high lactose content in the medium protects the Cys residue at or near the lactose-binding site from inactivation by NEM, possibly by sterically protecting it. You are unaware of any more information regarding the transporter protein. Suggestions for experiments that might be used to determine the Mr of this Cys-containing transporter polypeptide
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A Cys residue is required for the transport function of a bacterial lactose transporter that is highly selective for lactose. The transporter is permanently inactivated by the covalent interaction of N-ethylmaleimide (NEM) with this Cys residue. A high lactose content in the medium protects the Cys residue at or near the lactose-binding site from inactivation by NEM, possibly by sterically protecting it. You are unaware of any more information regarding the transporter protein. Suggestions for experiments that might be used to determine the Mr of this Cys-containing transporter polypeptide
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