The following quote is from a TiBS review article (Y. Shiloh,(2006) TiBS vol. 31 No7 Pages 402-410). Please explain what information would have been derived from genetics experiments and what information would have been based on biochemistry experiments. Explain how using both genetic and biochemistry has allowed us to greatly enhance our understanding of this field. ATM belongs to a conserved family of proteins termed the ‘PI3K-like protein kinases’( PIKKs), most of which possess a serine/threonine kinase activity and all of which, as their name indicates, contain a domain with motifs that are typical of the lipid kinase phosphatidylinositol3- kinase(PI3K) [39]. At present, the mammalian members of this family include five protein kinases: ATM, ataxia-telangiectasia- and Rad3-related (ATR), hSMG-1, mTOR (also known as FRAP) and the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). Another member of the PIKK family is TRRAP, a protein component of histone acetyltransferase complexes that does not process protein kinase activity [3, 39]. The PIKK protein kinases, which are conserved from yeast to mammals, respond to various stresses by phosphorylating substrates in the appropriate pathways.
The following quote is from a TiBS review article (Y. Shiloh,(2006) TiBS vol. 31 No7 Pages 402-410). Please explain what information would have been derived from genetics experiments and what information would have been based on biochemistry experiments. Explain how using both genetic and biochemistry has allowed us to greatly enhance our understanding of this field.
ATM belongs to a conserved family of proteins termed the ‘PI3K-like protein kinases’( PIKKs), most of which possess a serine/threonine kinase activity and all of which, as their name indicates, contain a domain with motifs that are typical of the lipid kinase phosphatidylinositol3- kinase(PI3K) [39]. At present, the mammalian members of this family include five protein kinases: ATM, ataxia-telangiectasia- and Rad3-related (ATR), hSMG-1, mTOR (also known as FRAP) and the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). Another member of the PIKK family is TRRAP, a protein component of histone acetyltransferase complexes that does not process protein kinase activity [3, 39]. The PIKK protein kinases, which are conserved from yeast to mammals, respond to various stresses by phosphorylating substrates in the appropriate pathways.
Step by step
Solved in 5 steps
