r DNA isolation using Qiagen extraction kit, the ophotometric measurements. Explain the situation i) The absorbance at 260 nm is higher than absort

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Explain the principles behind DNA isolation using silica membrane spin column from Qiagen.
2 After DNA isolation using Qiagen extraction kit, the final flow through was subjected to
spectrophotometric measurements. Explain the situation if the absorbances were found to be:
i) The absorbance at 260 nm is higher than absorbance at 280 nm?
ii) The absorbance at 230 is higher than absorbance at 260 nm?
Transcribed Image Text:Explain the principles behind DNA isolation using silica membrane spin column from Qiagen. 2 After DNA isolation using Qiagen extraction kit, the final flow through was subjected to spectrophotometric measurements. Explain the situation if the absorbances were found to be: i) The absorbance at 260 nm is higher than absorbance at 280 nm? ii) The absorbance at 230 is higher than absorbance at 260 nm?
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