Question 10: What does the presence of the prototrophic strain "control" for? In other words, what was its purpose in the experiment? To ensure that the UMM basic medium can support growth and to give an indication of what growth on such a medium should look like. To ensure that the control strain will grow in a rich environment which will give an idea of what mutant strains will look like on an UMM basic environment. To see if all other strains will grow in a rich environment. O There is no reason other than curiosity. To see if the control strain is prototrophic.

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### Experimental Design: Control Strain in Growth Media

**Question 10:** What does the presence of the prototrophic strain “control” for? In other words, what was its purpose in the experiment?

1. **To ensure that the UMM basic medium can support growth and to give an indication of what growth on such a medium should look like.**

2. **To ensure that the control strain will grow in a rich environment which will give an idea of what mutant strains will look like on an UMM basic environment.**

3. **To see if all other strains will grow in a rich environment.**

4. **There is no reason other than curiosity.**

5. **To see if the control strain is prototrophic.**

This image provides a multiple-choice question designed to assess the understanding of the purpose of a control strain in an experimental setup involving growth media. The correct answer to this question would help in the thorough understanding of the experimental design, especially in microbiology and genetics experiments, where control strains are essential for validating results. 

### Explanation:

In an experiment, various strains (mutant or wild-type) are often grown on different media to compare their growth patterns. Here's a detailed look at why the control strain is critical:

- **UMM basic medium**: This is a minimal medium that contains only the essential nutrients required for the growth of prototrophic organisms (those capable of synthesizing all their metabolites from basic nutrients).
- **Control Strain**: Typically a known strain that can grow under the provided basic conditions. It acts as a benchmark to ensure the medium is adequate for supporting growth.

The main point of including a prototrophic control is to guarantee that any growth differences observed in other strains are due to genetic variations and not because of medium inadequacies. This type of question is vital for students learning about experimental controls because it underscores the importance of having a standard for comparison in scientific experiments.
Transcribed Image Text:### Experimental Design: Control Strain in Growth Media **Question 10:** What does the presence of the prototrophic strain “control” for? In other words, what was its purpose in the experiment? 1. **To ensure that the UMM basic medium can support growth and to give an indication of what growth on such a medium should look like.** 2. **To ensure that the control strain will grow in a rich environment which will give an idea of what mutant strains will look like on an UMM basic environment.** 3. **To see if all other strains will grow in a rich environment.** 4. **There is no reason other than curiosity.** 5. **To see if the control strain is prototrophic.** This image provides a multiple-choice question designed to assess the understanding of the purpose of a control strain in an experimental setup involving growth media. The correct answer to this question would help in the thorough understanding of the experimental design, especially in microbiology and genetics experiments, where control strains are essential for validating results. ### Explanation: In an experiment, various strains (mutant or wild-type) are often grown on different media to compare their growth patterns. Here's a detailed look at why the control strain is critical: - **UMM basic medium**: This is a minimal medium that contains only the essential nutrients required for the growth of prototrophic organisms (those capable of synthesizing all their metabolites from basic nutrients). - **Control Strain**: Typically a known strain that can grow under the provided basic conditions. It acts as a benchmark to ensure the medium is adequate for supporting growth. The main point of including a prototrophic control is to guarantee that any growth differences observed in other strains are due to genetic variations and not because of medium inadequacies. This type of question is vital for students learning about experimental controls because it underscores the importance of having a standard for comparison in scientific experiments.
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