Using a random mutagenesis approach (transposon), you find a xylan degradation mutant in the bacterium *Prima exanimii*. This mutant has the observed phenotype when grown on xylan (open diamonds). You find that the transposon is located in the *xtrA* gene. The growth data and chromosomal region around the *xtrA* gene are shown below: ### Growth Data - **Graph**: The graph plots Optical Density (OD₆₀₀) against time (hours). - **Lines and Symbols**: - WT (wild type) strain: represented by closed circles, shows a steady increase in growth over time, peaking at around 1.0 OD₆₀₀. - ΔxtrA mutant (open diamonds): initial decrease in OD, then remains constant around 0.3 OD₆₀₀ after a short period. - xylA mutant (closed triangles): slight increase, then levels off around 0.4 OD₆₀₀. ### Chromosomal Region - Diagram shows four genes arranged in sequence: - *xtrC* → *xtrB* → *xtrA* → *xtrR* - Arrows indicate the direction of transcription. ### Questions **A.** What is the specific purpose for each of the two other strains shown in the growth experiment (those with black icons)? **B.** "The loss of the *xtrA* gene product is the cause of the growth defect." Do you agree with this interpretation of the data? Explain your answer. **C.** "The *xtrA* mutant strain has a reduced growth rate compared to the WT strain." Do you agree with this interpretation of the data? Explain your answer.
Using a random mutagenesis approach (transposon), you find a xylan degradation mutant in the bacterium *Prima exanimii*. This mutant has the observed phenotype when grown on xylan (open diamonds). You find that the transposon is located in the *xtrA* gene. The growth data and chromosomal region around the *xtrA* gene are shown below: ### Growth Data - **Graph**: The graph plots Optical Density (OD₆₀₀) against time (hours). - **Lines and Symbols**: - WT (wild type) strain: represented by closed circles, shows a steady increase in growth over time, peaking at around 1.0 OD₆₀₀. - ΔxtrA mutant (open diamonds): initial decrease in OD, then remains constant around 0.3 OD₆₀₀ after a short period. - xylA mutant (closed triangles): slight increase, then levels off around 0.4 OD₆₀₀. ### Chromosomal Region - Diagram shows four genes arranged in sequence: - *xtrC* → *xtrB* → *xtrA* → *xtrR* - Arrows indicate the direction of transcription. ### Questions **A.** What is the specific purpose for each of the two other strains shown in the growth experiment (those with black icons)? **B.** "The loss of the *xtrA* gene product is the cause of the growth defect." Do you agree with this interpretation of the data? Explain your answer. **C.** "The *xtrA* mutant strain has a reduced growth rate compared to the WT strain." Do you agree with this interpretation of the data? Explain your answer.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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