Using a random mutagenesis approach (transposon), you find a xylan degradation mutant in the bacterium Prima examinii. This mutant has the observed phenotype when grown on xylan (open diamonds). You find that the transposon is located in the xtrA gene. The growth data and chromosomal region around the xtrA gene are shown below: Growth Data Хуlan WT + xylA Chromosomal Region 08 06- xtrC xtrB xtrA xtrR 04 15 20 Time (hours) A. What is the specific purpose for each of the two other strains shown in the growth experiment (those with black icons)? B. "The loss of the xtrA gene product is the cause of the growth defect." Do you agree with this interpretation of the data? Explain your answer. C. "The xtrA mutant strain has a reduced growth rate compared to the WT strain." Do you agree with this interpretation of the data? Explain your answer.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Using a random mutagenesis approach (transposon), you find a xylan degradation mutant in the
bacterium Prima examinii. This mutant has the observed phenotype when grown on xylan (open
diamonds). You find that the transposon is located in the xtrA gene. The growth data and
chromosomal region around the xtrA gene are shown below:
Growth Data
Хуlan
WT
+ xylA
Chromosomal Region
08
06-
xtrC
xtrB
xtrA
xtrR
04
15
20
Time (hours)
A. What is the specific purpose for each of the two other strains shown in the growth experiment
(those with black icons)?
B. "The loss of the xtrA gene product is the cause of the growth defect." Do you agree with this
interpretation of the data? Explain your answer.
C. "The xtrA mutant strain has a reduced growth rate compared to the WT strain." Do you agree
with this interpretation of the data? Explain your answer.
Transcribed Image Text:Using a random mutagenesis approach (transposon), you find a xylan degradation mutant in the bacterium Prima examinii. This mutant has the observed phenotype when grown on xylan (open diamonds). You find that the transposon is located in the xtrA gene. The growth data and chromosomal region around the xtrA gene are shown below: Growth Data Хуlan WT + xylA Chromosomal Region 08 06- xtrC xtrB xtrA xtrR 04 15 20 Time (hours) A. What is the specific purpose for each of the two other strains shown in the growth experiment (those with black icons)? B. "The loss of the xtrA gene product is the cause of the growth defect." Do you agree with this interpretation of the data? Explain your answer. C. "The xtrA mutant strain has a reduced growth rate compared to the WT strain." Do you agree with this interpretation of the data? Explain your answer.
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