Q2 (a) In 2014, during their iGEM project on biodesalination, students were willing to modify bacteria so that instead of swimming they would float. To do this, they chose to firs knock-out one of the genes for motility (such as MotA) and then insert a gene sequence te make gas bubbles. They used the restriction enzyme Pstl, which recognizes the sequence 5'-CTGCAG-3' and cuts between A-G. (CTGCA/G). Using the sequence from one of the DNA strands of the gene below, 5'-TTGATTTTATCGCTGCAGTCGATTATCTGCGCCTGATTATCACTGCAGGCG-3' (fragment modified from the students' plasmid) (v) Provide the double strand sequences resulting from the action of Pstl.
Q2 (a) In 2014, during their iGEM project on biodesalination, students were willing to modify bacteria so that instead of swimming they would float. To do this, they chose to firs knock-out one of the genes for motility (such as MotA) and then insert a gene sequence te make gas bubbles. They used the restriction enzyme Pstl, which recognizes the sequence 5'-CTGCAG-3' and cuts between A-G. (CTGCA/G). Using the sequence from one of the DNA strands of the gene below, 5'-TTGATTTTATCGCTGCAGTCGATTATCTGCGCCTGATTATCACTGCAGGCG-3' (fragment modified from the students' plasmid) (v) Provide the double strand sequences resulting from the action of Pstl.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Q2
(a)
In 2014, during their iGEM project on biodesalination, students were willing to modify
bacteria so that instead of swimming they would float. To do this, they chose to first
knock-out one of the genes for motility (such as MotA) and then insert a gene sequence
te make gas bubbles. They used the restriction enzyme Pstl, which recognizes the
sequence 5'-CTGCAG-3' and cuts between A-G. (CTGCA/G). Using the sequence
from one of the DNA strands of the gene below,
5'-TTGATTTTATCGCTGCAGTCGATTATCTGCGCCTGATTATCACTGCAGGCG-3'
(fragment modified from the students' plasmid)
(v) Provide the double strand sequences resulting from the action of Pstl.
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