3. Today we examined the pattern of genomic DNA when it is cut by a restriction enzyme. One of the enzymes, EcoRI, recognizes a specific 6 bp sequence. How often would you expect this enzyme to cut a long stretch of DNA? About how many fragments does an EcoRI digest produce on the calf genome (Bos taurus, 3 x 10⁹ basepairs in length)? Show all work. [Hint: formula is (Length of genomic or plasmid DNA)*(1/4)^bp; where bp is the length of the restriction site. The reason for this is because there are 4 nucleotides. EcoRI recognizes GAATTC, so if we were to look at genomic DNA 6 bp at a time, there is a 4 chance of position 1 having a G, a ¼ chance of position 2 having an A, a 14 chance of position 3 having an A, and so on... ]
3. Today we examined the pattern of genomic DNA when it is cut by a restriction enzyme. One of the enzymes, EcoRI, recognizes a specific 6 bp sequence. How often would you expect this enzyme to cut a long stretch of DNA? About how many fragments does an EcoRI digest produce on the calf genome (Bos taurus, 3 x 10⁹ basepairs in length)? Show all work. [Hint: formula is (Length of genomic or plasmid DNA)*(1/4)^bp; where bp is the length of the restriction site. The reason for this is because there are 4 nucleotides. EcoRI recognizes GAATTC, so if we were to look at genomic DNA 6 bp at a time, there is a 4 chance of position 1 having a G, a ¼ chance of position 2 having an A, a 14 chance of position 3 having an A, and so on... ]
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Q3) plz
![3. Today we examined the pattern of genomic DNA when it is cut by a restriction enzyme. One of the enzymes,
EcoRI, recognizes a specific 6 bp sequence. How often would you expect this enzyme to cut a long stretch of
DNA? About how many fragments does an EcoRI digest produce on the calf genome (Bos taurus, 3 x 10⁹
basepairs in length)? Show all work.
[Hint: formula is (Length of genomic or plasmid DNA)*(1/4)^bp ; where bp is the length of the restriction site.
The reason for this is because there are 4 nucleotides. EcoRI recognizes GAATTC, so if we were to look at
genomic DNA 6 bp at a time, there is a ¼ chance of position 1 having a G, a ¼ chance of position 2 having an A,
a 14 chance of position 3 having an A, and so on... ]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcf8c917b-7d25-4fea-9b3f-90a987505563%2Fbd427d9e-83bb-46bb-a24a-816f78609d46%2F2q33j37_processed.png&w=3840&q=75)
Transcribed Image Text:3. Today we examined the pattern of genomic DNA when it is cut by a restriction enzyme. One of the enzymes,
EcoRI, recognizes a specific 6 bp sequence. How often would you expect this enzyme to cut a long stretch of
DNA? About how many fragments does an EcoRI digest produce on the calf genome (Bos taurus, 3 x 10⁹
basepairs in length)? Show all work.
[Hint: formula is (Length of genomic or plasmid DNA)*(1/4)^bp ; where bp is the length of the restriction site.
The reason for this is because there are 4 nucleotides. EcoRI recognizes GAATTC, so if we were to look at
genomic DNA 6 bp at a time, there is a ¼ chance of position 1 having a G, a ¼ chance of position 2 having an A,
a 14 chance of position 3 having an A, and so on... ]
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