Plz choose all correct option and Do explain. Which of the following descriptions about tandem affinity purification is not correct? a.The interaction between calmodulin-binding peptides and calmodulin beads can be broken by adding EDT b.In the first round of elution, TEV protease cleaves the cleavage site on the TAP-fused target protein. c.It enables the purification of protein complexes composed of multiple protein components. d.It is possible to perform two rounds of purification using a single TAP tag. e.The protein A tag binds to beads that have lgG attached to them.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Plz choose all correct option and Do explain.
Which of the following descriptions about tandem affinity purification is not correct?
a.The interaction between calmodulin-binding peptides and calmodulin beads can be broken by adding EDT
b.In the first round of elution, TEV protease cleaves the cleavage site on the TAP-fused target protein.
c.It enables the purification of protein complexes composed of multiple protein components.
d.It is possible to perform two rounds of purification using a single TAP tag.
e.The protein A tag binds to beads that have lgG attached to them.
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