3. One indication of the relative importance of various ATP-producing pathways is the Vmax of certainenzymes of these pathways. The values of Vmax of several enzymes from the pectoral muscles (chestmuscles used for flying) of pigeon and pheasant are listed below.Vmax (µmol substrate/min/g tissue)EnzymePigeonPheasantHexokinase3.02.3Glycogen phosphorylasePhosphofructokinase-1Citrate synthaseTriacylglycerol lipase18.0120.024.0143.0100.015.00.070.01d. Why were these enzymes selected for comparison? Would the activities of triose phosphate isomeraseand malate dehydrogenase be equally good bases for comparison? Explain.

The energy content of fat per gram is greater than that of glycogen. The tissue's ability to…

Answered: One indication of the relative…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

For the following enzymes (3-6) predict how the conditions will most likely affect the enzymes activity with one of the following and provide a 1 sentence explanation: a. increase activity b. decrease activity c. not likely to alter activity 3) alpha-ketoglutarate dehydrogenase complex binding to AMP when [AMP] is high. 4) phosphohexose isomerase binding NADH when [NADH] is high 5) phosphofructose-1 binding NAD+ when [NAD+] is high 6) pyruvate dehydrogenase complex binding to ATP when [ATP] is high
Draw the products of the reaction of xylulose-5-phosphate and erythrose-4-phosphate catalyzed by transketolase in the pentose phosphate pathway. Provide the structure in the protonation state found in physiological conditions. H H H OH FO HO-H H-OH H OPO3²- Q transketolase Draw glyceraldehyde-3- phosphate H H- H H H O OH OH OPO3²- Draw fructose-6- phosphate Q I I
Could you help propose a reasonable mechanism for the following biochemical transformation?
Using diagrams show the pathway that is used to convert succinate to phosphoenol-pyruvate, which can be used to make glucose through gluconeogenesis. Be sure to show the structure of all of the intermediates along the pathway and show the name of each enzyme over the arrow. Also be sure to indicate other co-substrates or cofactors required (ie. ATP, NAD+, FAD, etc…)
We have described an ATP synthase complex with an Fo complex of 9 c-subunits. Consider if the F0 complex had 12 c- subunits instead of only 9 (assume a eukaryote). What would be the P/O ratio (the ATP produced in the cytosol per atom of O reduced (or a pair of e- if you want to think of it that way) obtained from using B-hydroxy-fatty-acyl-CoA Dehydrogenase? (report to 1 decimal place) ATP
The following two reactions are coupled in the last step of glycolysis (catalyzed by the enzyme pyruvate kinase). РЕР + Н.О € Руr + Pi ADP + Pi → ATP + H2O AG° = -14.8 kcal/mol AG° = +7.3 kcal/mol D) The steady-state concentrations of ATP, ADP, and pyruvate in human erythrocytes are 2.24 mM, 0.25 mM, and 0.051 mM, respectively. What would be the concentration of PEP in these cells if the coupled reaction was at equilibrium?
Draw OUT THE arrow pushing reaction mechanism for the following steps of glycolysis: SHOW/IDENTIFY the arrow pushing mechanism in at least 2 steps. CH,OPO CH,OH hexokinase 2+ H H H H H H ОН + ATP OH H ADP + H+ H ОН + НО HO OH H ОН H ОН Glucose-6-phosphate (G6P) Glucose
The following Michaelis-Menten plot shows the response of phosphofructokinase-1 (PFK-1), an enzyme in the glycolytic pathway, to the presence of ATP and AMP. Based on the data below, which of the following statements apply? Select all that apply. No inhibitors (low (ATP)) 1 mM ATP + 0.1 mM AMP 1 mM ATP 1.0 2.0 (Fructose-6-phosphate) mM Fig. : Regulation of PFK activity AMP is an allosteric modifier that enhances the binding of the substrate fructose 6-phosphate а. O b. AMP is an allosteric modifier that reduces the binding of the substrate fructose 6-phosphate ATP is an allosteric modifier that reduces the binding of the substrate fructose 6-phosphate O c. O d. ATP is an allosteric modifier that enhances the binding of the substrate fructose 6-phosphate Phosphofructokinase activity
Otto Warburg made an interesting observation in the 1930s about cancer cells using the fermentation pathway to catabolize pyruvate even when the oxygen supply seemed sufficient for oxidative phosphorylation. One modern explanation for this “Warburg Effect” is that it protects the cancer cells from damage from free radicals. Provide a biochemical rationale for this explanation.
The enzyme pyruvate carboxylase is discovered in a bacterium that was thought not to contain it; in this case study, you’ll see how researchers study and characterize the enzyme, and, ultimately, show how it fits into a metabolic pathway. Following purification of the PYC enzyme by avidin-Sepharose affinity chromatography, the investigators carried out several experiments to characterize the enzyme. First, they ran samples of the enzyme on denaturing and non-denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where molecular weight standards of various sizes would occur if they’d been on the gels. The catalytic properties of the PYC enzyme were assessed following purification. The activity of the enzyme was assayed in the presence of ATP, pyruvate, bicarbonate…
This is a picture of the catalysis of alcohol by ADH with an inhibitor binding as an aldehyde analog. I have two questions. First, what would the enzyme be classified as and why? Also, what would the steps of this mechanism be?
An investigator briefly incubates an actively respiring bacterial culture with [1-14 C]glucose and isolates the glycolytic and citric acid cycle intermediates. Where is 14 C located in each of the intermediates listed? Consider only the initial incorporation of 14C in the first pass of labeled glucose through the pathways. fructose 1,6-bisphosphate glyceraldehyde 3-phosphate phosphoenolpyruvate acetyl-CoA citrate ☐ ☐ ☐ ☐ ☐ ☐ ☐ a-ketoglutarate oxaloacetate Answer Bank C-4 C-1 C-3 C-2

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS