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Biochemistry
Objective: The enzyme pyruvate carboxylase is discovered in a bacterium that was thought not to contain it; in this case study, you'll see how researchers study and characterize the enzyme, and, ultimately, show how it fits into a metabolic pathway. Note: You may need some of the information from Task 18 to complete this task. Following purification of the PYC enzyme by avidin-Sepharose affinity chromatography, the investigators carried out several experiments to characterize the enzyme. 6. First, they ran samples of the enzyme on denaturing and non- denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where 1 Non-denaturing Denaturing molecular weight standards of various sizes would occur if they'd been on the gels. Mwt Stds, KD 272 132 95 66 45 14 How many subunits are in the PYC enzyme? Are the subunits the same, or are they different in some way? Are the subunits made up of distinct chains of primary structures, and, if so, how many? Are the chains the same, or are they different in some way?

Objective: The enzyme pyruvate carboxylase is discovered in a bacterium that was thought not to contain it; in this case study, you'll see how researchers study and characterize the enzyme, and, ultimately, show how it fits into a metabolic pathway. Note: You may need some of the information from Task 18 to complete this task. Following purification of the PYC enzyme by avidin-Sepharose affinity chromatography, the investigators carried out several experiments to characterize the enzyme. 6. First, they ran samples of the enzyme on denaturing and non- denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where 1 Non-denaturing Denaturing molecular weight standards of various sizes would occur if they'd been on the gels. Mwt Stds, KD 272 132 95 66 45 14 How many subunits are in the PYC enzyme? Are the subunits the same, or are they different in some way? Are the subunits made up of distinct chains of primary structures, and, if so, how many? Are the chains the same, or are they different in some way?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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First, they ran samples of the enzyme on denaturing and non-denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where molecular weight standards of various sizes would occur if they’d been on the gels.

How many subunits are in the PYC enzyme? Are the subunits the same, or are they different in some way? Are the subunits made up of distinct chains of primary structures, and, if so, how many? Are the chains the same, or are they different in some way?

Objective: The enzyme pyruvate carboxylase is discovered in a bacterium that was thought not to contain it; in this case study, you'll see how researchers study and characterize the enzyme, and, ultimately, show how it fits into a metabolic pathway. Note: You may need some of the information from Task 18 to complete this task. Following purification of the PYC enzyme by avidin-Sepharose affinity chromatography, the investigators carried out several experiments to characterize the enzyme. 6. First, they ran samples of the enzyme on denaturing and non- denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where Lotto Mwt Stds, kD 272 132 95 66 molecular weight standards of various sizes would occur if they'd been on the gels. IMA || Non-denaturing Denaturing 45 14 How many subunits are in the PYC enzyme? Are the subunits the same, or are they different in some way? Are the subunits made up of distinct chains of primary structures, and, if so, how many? Are the chains the same, or are they different in some way?
Transcribed Image Text:Objective: The enzyme pyruvate carboxylase is discovered in a bacterium that was thought not to contain it; in this case study, you'll see how researchers study and characterize the enzyme, and, ultimately, show how it fits into a metabolic pathway. Note: You may need some of the information from Task 18 to complete this task. Following purification of the PYC enzyme by avidin-Sepharose affinity chromatography, the investigators carried out several experiments to characterize the enzyme. 6. First, they ran samples of the enzyme on denaturing and non- denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where Lotto Mwt Stds, kD 272 132 95 66 molecular weight standards of various sizes would occur if they'd been on the gels. IMA || Non-denaturing Denaturing 45 14 How many subunits are in the PYC enzyme? Are the subunits the same, or are they different in some way? Are the subunits made up of distinct chains of primary structures, and, if so, how many? Are the chains the same, or are they different in some way?
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