Most enzymatic reactions consume substrate or produce products which cannot be monitored directly with spectrophotometric methods because they do not absorb light. What can be done in this case to monitor the substrate consumption or product formation in order to calculate kinetic parameters? Group of answer choices   switch to a continuous assay   switch to a discontinuous assay   use a coupled reaction which converts the product into one that has a chromophore

Q: Agarose gel electrophoresis is relatively simple and straight forward to perform. Agarose is a…

A: two monomer units present in agarose are:

Q: None

A: Lineweaver-Burk Plot Basics:The Lineweaver-Burk plot is a graph that helps understand enzyme…

Q: 28. If an enzyme with a Vmax of 4.3 µmol.min', and a Km of 150 µM was investigated under pre-set…

A: The Michaelis- Menten equation helps in estimating the relation between the rate of the reaction and…

Q: For an enzymatic assay, tumour cells are incubated at different concentrations in individual vials…

A: The explanation is given below-

Q: This activity is an assignment. For the following two reactions, draw the possible products and if…

A: Step 1:Step 2: Step 3: Step 4:

Q: Draw a reaction coordinate diagram for the following mechanism. Your diagram should clearly indicate…

A:

Q: A research group discovers a new version of the rainbowase enzyme, which they call rainbowase*, that…

A: “Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Q: An enzyme has a Km of 8 M in the absence of an inhibitor and an apparent Km of 12 M in the presence…

A: Given Km = 8 M (in absence of inhibitor)  KmApp = 12 M (in presence of inhibitor) Inhibitor…

Q: This is an activity from the reading and will not be tested in this detail on the final exam (so…

A:

Q: None

A: EXPLANATION :Part 1 : Find expression of rate constant , rFirst of all in step one we have formed…

Q: Figure 7 of Conrad et al. (1999) shows the variation in C-isotopic composition of CO₂ as a function…

A: The objective of this question os to show the presented the variation of the isotopic composition of…

Q: An enzyme with Km value of 15.75 M and Vm is 30 micromole/L.min is used in a batch reactor.…

A: Km = 15.75 M or mol/L Vmax=  30 μmol/L.min Conversion, X = 88 % Initial concentration of substrate =…

Q: During a Michaelis Menten Kinetics experiment the following the standard curve and absorbance of one…

A: A question based on kinetics of reaction in solution that is to be accomplished.

Q: Don't use ai i will report you answer solve it as soon as possible with proper explanation

A:

Q: rate law expression is R=K[i-J][H2O2[H+] because all my integers were 0, 0, 0. in my instructions…

A: Rate law is written in terms of concentration and order of reactants.

Q: 3. The development step • Improve pharmacokinetic properties (ADME) > Toxicological evaluation •…

A: The pharmokinetical properties are the properties that are used to characterize the absorption of…

Q: Chemistry suggest mechanism of Photoinduced Electron Transfer Reaction

A: In the given problem we need to find the mechanism of the photoinduced electron transfer reaction.

Q: INITIAL INVESTIGATION Run kinetics experiments on XYZase without inhibitors and with compound X.…

A: The objective of the question is to determine whether XYZase follows Michaelis-Menten kinetics, to…

Q: What is the value in kJ/mol, of the lowest energy trnasition above?  a.) 545 b.) 444 c.) 320 d.)…

A: The absorbance v/s wavelength curve given is,

Q: In the cases where binding potency of the ligand A is determined in a functional assay in the…

A: The question is asking to define the relationship between EC50 for a ligand A and KD for a molecule…

Q: The enzyme reaction follows the Michaelis-Menten kinetic and the relative reaction rate (V/Vmax) has…

A:

Q: help please answer in text form with proper workings and explanation for each and every part and…

A: Approach to solving the question:Read and understand the question.Analyze the provided image.Outline…

Q: the mechanism for when salicylic acid reacts with p-toluidine. here's what was done for the…

A: When benzoic acid is treated with thionyl chloride it form the benzoyl chloride. Then it is used for…

Q: Determine which of the substrates will and will not react with NaOMeNaOMe in an SN2SN2 reaction to…

A: In SN2 reaction the nucleophile attacks the primary or secondary alkyl halide and gives the…

Q: Provide a multistep synthesis.

A: The above transformation involves reduction of ester to form aldehyde, methylation at alpha position…

Q: What is the structure of the active electrophile formed in a peptide coupling mediated by…

A: Therefore correct option is second structure. DCC=dicyclohexylcarbodiimide is a coupling reagent for…

Q: 4. (a) If we have an enzyme solution whose concentration is 1.0 x 10“ M and with a keat value of…

A: We can solve this problem by using the Michaelis-Menten equation  Vo= (Vmax × [S])/(Vm+[S]) Vo =…

Q: Which of the following Lineweaver-Burk parameters does not change when inhibitor X is added to the…

A: The Lineweaver–Burk plot can distinguish inhibitors (competitive, non-competitive and…

Q: Explain the principles of Enzymatic Kinetic Resolution and use an example and a diagram to support…

A:

Q: Using the attached target molecule and synthesis, please state (in words) what the starting…

A: The given reaction proceeds through Diels-Alder reaction of diene and dienophile to form an…

Q: Q7. Kinetics were analysed for an enzyme in the absence and presence of inhibitor. In the absence of…

A: A multiple choice question based on kinetics of reactions that is to be accomplished.

Q: t's try similar things with another enzyme! Here's one with a cool mechanism that makes an eresting…

A: Applying concept of type of chemical reaction.

Q: Question 15 Plots A and B show enzyme kinetics data in the absence and presence of inhibitor. Which…

A:

Q: i need help with this question asap

A:

Q: meo

A:

Q: After taking Anemovax vaccine, it is recommended that the booster dose be Dendrovax. The combination…

A: Given:

Q: b) Adsorption of N₂ on TiO₂ was carried out at 75K. A plot of Z/(1-Z)V versus Z (Z-P/P®) gives a…

A: Since you have asked multiple questions we will solve first question for you.Brunauer–Emmett–Teller…

Mass Spectrometry

This process is an instrumental method in which a sample is changed to fast moving positive ions by electron bombardment. The charged particles vary due to their different mass to charge ratio and are estimated with the help of this difference. It is also called mass spectroscopy.