A research group discovers a new version of the rainbowase enzyme, which they call rainbowase*, that catalyzes the chemical reaction:\[ \text{RAIN} \rightleftharpoons \text{rainbows} \]The researchers began to characterize the enzyme. a) In the 1st experiment, with \([\text{E}_t]\) at 4 nM, they find that the \( V_{\text{max}} \) is 1.6 μM s⁻¹. Based on this experiment, what is the kcat for rainbowase*? (include units and show work).b) In another experiment, the \([\text{E}_t]\) at 1 nM and \([\text{RAIN}]\) at 30 μM, the researchers find that \( V_0 = 300 \) nM s⁻¹. What is the measure \( K_M \) for rainbowase* for its substrate RAIN? (include units and show work). c) Further research shows that the purified rainbowase* used in the first two experiments was actually contaminated with a reversible inhibitor called “NO RAIN, NO rainbows”. When “NO RAIN, NO rainbows” is carefully removed from the rainbowase* preparation, and the two experiments are repeated, the measured \( V_{\text{max}} \) in (a) is increased to 4.8 µM\(^{-1}\), and the measured \( K_M \) is (b) now 15 µM. For the inhibitor “NO RAIN, NO rainbows”, calculate the values of α and α’.d) Based on the information given above, what type of inhibitor is “NO RAIN, NO rainbows”? (competitive, uncompetitive, mixed inhibitor, or other)?

“Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

A research group discovers a new version of the rainbowase enzyme, which they call rainbowase*, that catalyzes the chemical reaction: RAIN rainbows The researchers began to characterize the enzyme. a) In the 1st experiment, with [E] at 4 nM, they find that the Vmax is 1.6 µM s-1. Based on this experiment, what is the kcat for rainbowase*? (include units and show work).

A research group discovers a new version of the rainbowase enzyme, which they call rainbowase, that catalyzes the chemical reaction: RAIN rainbows The researchers began to characterize the enzyme. a) In the 1st experiment, with [E] at 4 nM, they find that the Vmax is 1.6 µM s-1. Based on this experiment, what is the kcat for rainbowase? (include units and show work).

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

I. A research group discovers a novel enzyme called happiness synthetase that catalyzes the reaction: SAD → HAPPY a) In one kinetics experiment, with enzyme concentration [E]T at 0.04 µM, the researchers find that the Vmax is 1.6 µM/s. Based on the experiment and assuming Michaelis-Menten kinetics applies, what is the turnover number for the enzyme? b) Another name for turnover number is: Kon Koff c) What happens to the Vmax for the enzyme increases decreases Keat Kd KM reaction when you add more enzyme? does not change d) In another experiment, with substrate concentration [SAD] at 30 µM, the researchers find that Vo = 0.40 μM/s. What is the measured KM of the happiness synthetase enzyme for SAD? (Use the same Vmax and [E]T values noted in (a)). e) What happens to the KM for the enzyme-substrate pair when you add more enzyme? decreases does not change increases f) What is the catalytic efficiency of the happiness synthetase enzyme with the SAD substrate? (Note: 1 µM = 1 x 106 M) g)…
a Using the energy diagram that follows, estimate the activation energy and the heat of reaction. Energy (kcal) 35 30 25 20 15 10 5 0 Progress of reaction (Enter your answer to two significant figures.) Activation energy: Heat of the reaction: kcal kcal
::/((/(/(/(///
5-6
# 3 E D C Energy diagrams for two reactions are shown. Macmillan Learning Energy (kJ/mol) e 150 100 50- What is the heat of reaction for Reaction A? AHrxnA = 4 FER 20 What is the activation energy for Reaction A? R F V Reaction progress Reaction A 5 T G Search or type URL G B tv 6 MacBook Pro Y Ⓒ H 201 7 N U S J kJ mol 00 8 M I Energy (kJ/mol) 150 T 100- NIMZAO ✪ 50 What is the heat of reaction for Reaction B? AHxnB = What is the activation energy for Reaction B? K ( - O 9 < O I * ) -C O Reaction progress Reaction B L command ») P . V = : P W ; 242) { option [ SEC e = ? O kJ mol 1 delete retu
1:34 PM Sun Feb 5 < Question 30 of 31 The activation energy, Ea, for a particular reaction is 37.8 kJ/mol. If the rate constant at 280 K is 0.178 M/s, then what is the value of the rate constant at 429 K? (R = 8.314 J/mol K) ● Tap here or pull up for additional resources
( asap within 10 min or eslse dislike asp plzz 10 min fast )
The Ksp equilibrium expression for lead(II) chloride is Pb₂Cl(s) 2Pb²+ (aq) + Cl¹-(aq). True False
Background Layout Theme Transition 11|2|3|4|56|17|T Number Reactants Yield Products 1 2 Mg= 0= did speaker notes Ca= 0= Mg+ 0₂ Ca+ & ↑ Mg = 0= Ca = 0= Mg0 CaO 891 MASHAR +
At pH = 7, the measured Michaelis constant and maximum velocity for the enzymatic conversion of fumarate to L-malate, Fumarate + H2O → L-Malate are 4.0 x 10-6 M and 1.3 x 10 3 [E]0 s-1 , respectively, where [E]0 is the total molar concentration of the enzyme. The Michaelis constant and maximum velocity for the reverse reaction are 1.0 x 10-5 M and 800 [E]0 s-1 , respectively. 8A)WHAT is the equilibrium constant (a value) for the hydration reaction? [Notes: the arrow above is an equilibrium arrow. The activity of water is set equal to unity] 8B.) DRAW all chemical entities in the forward, balanced chemical reaction, above.
EXAMPLE Age of a Fossil A fossilized bone is found to contain 0.1% of its original amount of C-14. Determine the age of the fossil. SOLUTION The starting point is again A(t) = Aoekt. To determine the value of the decay constant k we use the fact that Ao = A(5730) or Ao = Age 5730k. The last equation implies 5730k = In-In2 and so we get k = -(In2)/5730 = -0.00012097. Therefore A(t) = Age-0.00012097, With A(t) = 0.001Ao we have 0.001A0 = Age-0.000120971 and -0.00012097t=In(0.001) -In 1000. Thus = t= In 1000 0.00012097 57,100 years.
8. Which factor would slow down a chemical reaction? (Choose all that apply) Increasing particle size Decreasing particle size U Adding an inhibitor Decreasing temperature