A research group discovers a new version of the rainbowase enzyme, which theycall rainbowase*, that catalyzes the chemical reaction:RAINrainbowsThe researchers began to characterize the enzyme.a) In the 1st experiment, with [E] at 4 nM, they find that the Vmax is 1.6 uM s-1. Based on thisexperiment, what is the kcat for rainbowase*? (include units and show work).b) In another experiment, the [E] at 1 nM and [RAIN] at 30 µM, the researchers find that V.= 300 nM s-1. What is the measure KM for rainbowase* for its substrate RAIN? (includeunits and show work). c) Further research shows that the purified rainbowase* used in the first two experimentswas actually contaminated with a reversible inhibitor called "NO RAIN, NO rainbows".When “NO RAIN, NO rainbows" is carefully removed from the rainbowase* preparation,and the two experiments are repeated, the measured Vmax in (a) is increased to4.8 µM -1, and the measured KM is (b) is now 15 µM. For the inhibitor "NO RAIN, NOrainbows", calculate the values of a and a'.d) Based on the information given above, what type of inhibitor is "NO RAIN, NO rainbows"?(competitive, uncompetitive, mixed inhibitor, or other)?

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A research group discovers a new version of the rainbowase enzyme, which they call rainbowase*, that catalyzes the chemical reaction: RAIN rainbows The researchers began to characterize the enzyme. a) In the 1st experiment, with [E] at 4 nM, they find that the Vmax is 1.6 µM s-1. Based on this experiment, what is the kcat for rainbowase*? (include units and show work).

A research group discovers a new version of the rainbowase enzyme, which they call rainbowase, that catalyzes the chemical reaction: RAIN rainbows The researchers began to characterize the enzyme. a) In the 1st experiment, with [E] at 4 nM, they find that the Vmax is 1.6 µM s-1. Based on this experiment, what is the kcat for rainbowase? (include units and show work).

Chemistry for Engineering Students

3rd Edition

ISBN:9781285199023

Author:Lawrence S. Brown, Tom Holme

Publisher:Lawrence S. Brown, Tom Holme

Chapter11: Chemical Kinetics

Section: Chapter Questions

Problem 11.61PAE

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A research group discovers a neW version oI happyasee, which they nappyase", that catalyzes the chemical reaction HAPPY = SAD The researchers begin to characterize the enzyme. In the first experiment, with [E] at 4 nM, they find that the Vmax is 1.6 µM s-1. Based on this experiment, what is the kcat for happyase*? kcat 400 S In another experiment, with [E,] at 1 nM and [HAPPY] at 30 µM, the researchers find that Vo is equal to 300 nM s-1. What i the measured Km of happyase* for its substrate HAPPY? Km 10 x10–6 Incorrect Further research shows that the purified happyase* used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation, and the two experiments repeated, the measured Vmax increases to 4.8 µM s-, and the measured Km is now 15 µM. S For the inhibitor ANGER, calculate the values of a' and a. a' 2 Incorrect 2
A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction The researchers begin to characterize the enzyme. a)In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 μM s-1. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.) b)In another experiment, with [Et] at 1 nM and [HAPPY] at 30 μM, the researchers find that V0300 nM s-1. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.) c)Further research shows that the purified happyase* used in the first two experiments wasactually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation, and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 μM s-1, and the measured Km in (b) is now 15 μM. For the inhibitor ANGER, calculate the values of αand α’.
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A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction HAPPY ⇌ SAD. The researchers begin to characterize the enzyme. (a) In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 μM s−1. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.) (b) In another experiment, with [Et] at 1 nM and [HAPPY] at 30 μM, the researchers find that V0 = 300 nM s−1. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.) (c) Further research shows that the purified happyase used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase preparation and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 μM s−1, and the measured Km in (b) is now 15 μM. For the inhibitor ANGER, calculate the values of α and α′. . (d) Based on the information given…
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حد يدبرلنا طريقة حل فرع C A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction HAPPY ⇌ SAD. The researchers begin to characterize the enzyme. (a) In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 μM s−1. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.) (b) In another experiment, with [Et] at 1 nM and [HAPPY] at 30 μM, the researchers find that V0 = 300 nM s−1. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.) (c) Further research shows that the purified happyase used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase preparation and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 μM s−1, and the measured Km in (b) is now 15 μM. For the inhibitor ANGER, calculate the values of α and α′. . (d)…
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