I. A research group discovers a novel enzyme called happiness synthetase that catalyzes the reaction:SAD → HAPPYa) In one kinetics experiment, with enzyme concentration [E]T at 0.04 µM, the researchers find that theVmax is 1.6 µM/s. Based on the experiment and assuming Michaelis-Menten kinetics applies, what is theturnover number for the enzyme?b) Another name for turnover number is: Kon Koffc) What happens to the Vmax for the enzymeincreasesdecreasesKeat Kd KMreaction when you add more enzyme?does not changed) In another experiment, with substrate concentration [SAD] at 30 µM, the researchers find thatVo = 0.40 μM/s. What is the measured KM of the happiness synthetase enzyme for SAD?(Use the same Vmax and [E]T values noted in (a)).e) What happens to the KM for the enzyme-substrate pair when you add more enzyme?decreasesdoes not changeincreasesf) What is the catalytic efficiency of the happiness synthetase enzyme with the SAD substrate?(Note: 1 µM = 1 x 106 M)g) Based on your answer in (f), will you consider happiness synthetase as a kinetically perfect andefficient enzyme that is diffusion-limited? Explain your answer.

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I. A research group discovers a novel enzyme called happiness synthetase that catalyzes the reaction: SAD → HAPPY a) In one kinetics experiment, with enzyme concentration [E]T at 0.04 µM, the researchers find that the Vmax is 1.6 µM/s. Based on the experiment and assuming Michaelis-Menten kinetics applies, what is the turnover number for the enzyme? b) Another name for turnover number is: Kon Koff c) What happens to the Vmax for the enzyme increases decreases Keat Kd KM reaction when you add more enzyme? does not change

I. A research group discovers a novel enzyme called happiness synthetase that catalyzes the reaction: SAD → HAPPY a) In one kinetics experiment, with enzyme concentration [E]T at 0.04 µM, the researchers find that the Vmax is 1.6 µM/s. Based on the experiment and assuming Michaelis-Menten kinetics applies, what is the turnover number for the enzyme? b) Another name for turnover number is: Kon Koff c) What happens to the Vmax for the enzyme increases decreases Keat Kd KM reaction when you add more enzyme? does not change

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

I. A research group discovers a novel enzyme called happiness synthetase that catalyzes the reaction:
SAD → HAPPY
a) In one kinetics experiment, with enzyme concentration [E]T at 0.04 µM, the researchers find that the
Vmax is 1.6 µM/s. Based on the experiment and assuming Michaelis-Menten kinetics applies, what is the
turnover number for the enzyme?
b) Another name for turnover number is: Kon Koff
c) What happens to the Vmax for the enzyme
increases
decreases
Keat Kd KM
reaction when you add more enzyme?
does not change
d) In another experiment, with substrate concentration [SAD] at 30 µM, the researchers find that
Vo = 0.40 μM/s. What is the measured KM of the happiness synthetase enzyme for SAD?
(Use the same Vmax and [E]T values noted in (a)).
e) What happens to the KM for the enzyme-substrate pair when you add more enzyme?
decreases
does not change
increases
f) What is the catalytic efficiency of the happiness synthetase enzyme with the SAD substrate?
(Note: 1 µM = 1 x 106 M)
g) Based on your answer in (f), will you consider happiness synthetase as a kinetically perfect and
efficient enzyme that is diffusion-limited? Explain your answer.

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