I. A research group discovers a novel enzyme called happiness synthetase that catalyzes the reaction:SAD → HAPPYa) In one kinetics experiment, with enzyme concentration [E]T at 0.04 µM, the researchers find that theVmax is 1.6 µM/s. Based on the experiment and assuming Michaelis-Menten kinetics applies, what is theturnover number for the enzyme?b) Another name for turnover number is: Kon Koffc) What happens to the Vmax for the enzymeincreasesdecreasesKeat Kd KMreaction when you add more enzyme?does not changed) In another experiment, with substrate concentration [SAD] at 30 µM, the researchers find thatVo = 0.40 μM/s. What is the measured KM of the happiness synthetase enzyme for SAD?(Use the same Vmax and [E]T values noted in (a)).e) What happens to the KM for the enzyme-substrate pair when you add more enzyme?decreasesdoes not changeincreasesf) What is the catalytic efficiency of the happiness synthetase enzyme with the SAD substrate?(Note: 1 µM = 1 x 106 M)g) Based on your answer in (f), will you consider happiness synthetase as a kinetically perfect andefficient enzyme that is diffusion-limited? Explain your answer.

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I. A research group discovers a novel enzyme called happiness synthetase that catalyzes the reaction: SAD → HAPPY a) In one kinetics experiment, with enzyme concentration [E]T at 0.04 µM, the researchers find that the Vmax is 1.6 µM/s. Based on the experiment and assuming Michaelis-Menten kinetics applies, what is the turnover number for the enzyme? b) Another name for turnover number is: Kon Koff c) What happens to the Vmax for the enzyme increases decreases Keat Kd KM reaction when you add more enzyme? does not change

I. A research group discovers a novel enzyme called happiness synthetase that catalyzes the reaction: SAD → HAPPY a) In one kinetics experiment, with enzyme concentration [E]T at 0.04 µM, the researchers find that the Vmax is 1.6 µM/s. Based on the experiment and assuming Michaelis-Menten kinetics applies, what is the turnover number for the enzyme? b) Another name for turnover number is: Kon Koff c) What happens to the Vmax for the enzyme increases decreases Keat Kd KM reaction when you add more enzyme? does not change

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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