The enzyme examase catalyses the following reaction, A - B where A is the reactant and B is the product A595 measurements were used to determine the catalytic activity of examase by measuring the rate at which B is produced. From this, the activity of the enzyme was determined to be 42 µmol/mL per minute. A standard albumin solution (concentration = 10 mg/mL) was used to prepare a standard curve of Abs595 vs amount of protein in mg. The protein assay was also performed using 100 ul of a 1 in 10 dilution of the original examase sample. For the absorbance measurements, it was determined that the sample used in the A595 measurement contains 1.2 mg of examase. Which of the following statements correctly gives the specific activity of examase? 1.0.35 U/mg O 2.0.035 U/mg O 3. 5040 U/mg O 4.35 U/mg O 5.3.5 U/mg
The enzyme examase catalyses the following reaction, A - B where A is the reactant and B is the product A595 measurements were used to determine the catalytic activity of examase by measuring the rate at which B is produced. From this, the activity of the enzyme was determined to be 42 µmol/mL per minute. A standard albumin solution (concentration = 10 mg/mL) was used to prepare a standard curve of Abs595 vs amount of protein in mg. The protein assay was also performed using 100 ul of a 1 in 10 dilution of the original examase sample. For the absorbance measurements, it was determined that the sample used in the A595 measurement contains 1.2 mg of examase. Which of the following statements correctly gives the specific activity of examase? 1.0.35 U/mg O 2.0.035 U/mg O 3. 5040 U/mg O 4.35 U/mg O 5.3.5 U/mg
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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
Transcribed Image Text:The enzyme examase catalyses the following reaction,
A - B
where A is the reactant and B is the product
A595 measurements were used to determine the catalytic activity of examase by measuring the rate at
which B is produced. From this, the activity of the enzyme was determined to be 42 µmol/mL per minute.
A standard albumin solution (concentration = 10 mg/mL) was used to prepare a standard curve of Abs595
vs amount of protein in mg. The protein assay was also performed using 100 uL of a 1 in 10 dilution of the
original examase sample. For the absorbance measurements, it was determined that the sample used in
the A595 measurement contains 1.2 mg of examase.
Which of the following statements correctly gives the specific activity of examase?
1.0.35 U/mg
O 2.0.035 U/mg
3. 5040 U/mg
O 4.35 U/mg
O 5.3.5 U/mg
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