Acetylcholinesterase is an enzyme that catalyses the hydrolysis of acetylcholine to choline. After several purification methods,  44.0 µg of acetylcholinesterase was purified from a crude extract and was found to catalyse the conversion of 16.0 μmol of acetylcholine (the substrate) to choline (the product) in 9 mins. What is the specific activity of acetylcholinesterase in units μmol min-1 mg-1.

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
icon
Related questions
icon
Concept explainers
Question

Acetylcholinesterase is an enzyme that catalyses the hydrolysis of acetylcholine to choline. After several purification methods,  44.0 µg of acetylcholinesterase was purified from a crude extract and was found to catalyse the conversion of 16.0 μmol of acetylcholine (the substrate) to choline (the product) in 9 mins. What is the specific activity of acetylcholinesterase in units μmol min-1 mg-1.  

(Round your answer to the nearest whole number. (i.e. If you calculated 40.03, input your answer as 40. Input only the numerical value. Do not input the unit).

Expert Solution
Step 1: Given data

Provided, 

  • amount of acetylcholinesterase purified from the crude extract is 44.0 μg.

  • The amount of substrate (acetylcholine) converted by the enzyme is 16.0 μmol.

  • The time it took for this conversion to occur was 9 minutes.

        For specific activity of acetylcholinesterase in units of bold italic mu bold italic m bold italic o bold italic l bold italic divided by bold italic m bold italic i bold italic n bold italic divided by bold italic m bold italic g bold italic comma we can use the following formula  bold italic S bold italic p bold italic e bold italic c bold italic i bold italic f bold italic i bold italic c bold italic space bold italic A bold italic c bold italic t bold italic i bold italic v bold italic i bold italic t bold italic y bold italic space bold italic left parenthesis bold italic mu bold italic m bold italic o bold italic l bold italic. bold italic m bold italic i bold italic n to the power of bold italic minus bold italic 1 end exponent bold italic. bold italic m bold italic g to the power of bold italic minus bold italic 1 end exponent bold italic right parenthesis bold italic space bold italic equals bold italic space fraction numerator bold italic left parenthesis bold italic A bold italic m bold italic o bold italic u bold italic n bold italic t bold italic space bold italic o bold italic f bold italic space bold italic P bold italic r bold italic o bold italic d bold italic u bold italic c bold italic t bold italic space bold italic f bold italic o bold italic r bold italic m bold italic e bold italic d bold italic space bold italic i bold italic n bold italic space bold italic mu bold italic m bold italic o bold italic l bold italic right parenthesis over denominator bold italic space bold italic left parenthesis bold italic T bold italic i bold italic m bold italic e bold italic space bold italic i bold italic n bold italic space bold italic m bold italic i bold italic n bold italic u bold italic t bold italic e bold italic s bold italic space bold italic cross times bold italic A bold italic m bold italic o bold italic u bold italic n bold italic t bold italic space bold italic o bold italic f bold italic space bold italic E bold italic n bold italic z bold italic y bold italic m bold italic e bold italic space bold italic i bold italic n bold italic space bold italic m bold italic g bold italic right parenthesis end fraction bold italic rightwards arrow bold italic left parenthesis bold italic 1 bold italic right parenthesis


steps

Step by step

Solved in 3 steps with 5 images

Blurred answer
Knowledge Booster
Catalysis and Enzymatic Reactions
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY