11. Applying the Michaelis-Menten Equation III Aresearch group discovers a new version of happyase, which they call hap- pyase, that catalyzes the chemical reaction HAPPY SAD. The researchers begin to characterize the erzyme. (a) In the first experiment, with [E) at 4 nM, they find that the V is 1.6 JM s. Based on this experiment, what is the kat for happyase*? (Include appropriate units.) (b) In another experiment, with [E at I nM and (HAPPY) at 30 juM, the researchers find that V= 300 nM s What is the measured K of happyase for its substrate HAPPY? (Include appropriate units.) (c) Further research shows that the purified happyase used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase" preparation and the two experiments repeated, the measured Vas in (a) is in- creased to 4.8 uM S AM. For the inhibitor ANGER, calculate the values of a and a', (d) Based on the information given above, what type of %3D and the measured K in (b) is now 15 inhihitor is ANGER?

11. Applying the Michaelis-Menten Equation III Aresearch group discovers a new version of happyase, which they call hap- pyase, that catalyzes the chemical reaction HAPPY SAD. The researchers begin to characterize the erzyme. (a) In the first experiment, with [E) at 4 nM, they find that the V is 1.6 JM s. Based on this experiment, what is the kat for happyase*? (Include appropriate units.) (b) In another experiment, with [E at I nM and (HAPPY) at 30 juM, the researchers find that V= 300 nM s What is the measured K of happyase for its substrate HAPPY? (Include appropriate units.) (c) Further research shows that the purified happyase used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase" preparation and the two experiments repeated, the measured Vas in (a) is in- creased to 4.8 uM S AM. For the inhibitor ANGER, calculate the values of a and a', (d) Based on the information given above, what type of %3D and the measured K in (b) is now 15 inhihitor is ANGER?

Chemistry & Chemical Reactivity

10th Edition

ISBN:9781337399074

Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Chapter14: Chemical Kinetics: The Rates Of Chemical Reactions

Section14.7: Reaction Mechanisms

Problem 1.2ACP

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Concept explainers

Catalysis and Enzymatic Reactions

Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.

Lock And Key Model

The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.

Question

11. Applying the Michaelis-Menten Equation III Aresearch
group discovers a new version of happyase, which they call hap-
pyase*, that catalyzes the chemical reaction HAPPY = SAD.
The researchers begin to characterize the enzyme.
(a) In the first experiment, with [E] at 4 nM, they find that
the Vnux is 1.6 M s, Based on this experiment, what is the
kat for happyase*? (Include appropriate units.)
(b) In another experiment, with [E] at 1 nM and [HAPPY]
at 30 LM, the researchers find that V, = 300 IM s. What is the
measured K of happyase* for its substrate HAPPY? (Include
appropriate units.)
(c) Further research shows that the purified happyase*
used in the first two experiments was actually contaminated
with a reversible inhibitor called ANGER. When ANGER is
carefully removed from the happyase* preparation and the
two experiments repeated, the measured Vmax in (a) is in-
creased to 4.8 uM S,
pIM. For the inhibitor ANGER, calculate the values of a and o
(d) Based on the information given above, what type of
and the measured Km in (b) is now 15
inhibitor is ANGER?
Fouation IV An

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