INITIAL INVESTIGATION Run kinetics experiments on XYZase without inhibitors and with compound X. Results: The compound found in the coffee (compound X) has yet to be identified, but using the small amount extracted, you have discovered that it is an inhibitor of XYZase. Given this revelation, it seems clear that this compound is the most likely murder weapon. Shown below is the data you have gathered. Note: [S] is given in nM; other values represent observed Vo values given in units of mol product formed per minute. Vo (no inhibitor) [S] (nM) 50 75 150 400 yes From the limited data you have gathered, does it appear that XYZase follows Michaelis-Menten kinetics? no 0.14 0.19 0.32 0.47 6.3 Vo (Cmpd X) 0.040 0.050 0.080 0.14 What is the Km for XYZase within two significant figures? Incorrect 190 Incorrect Assuming that the experiments performed in the absence of inhibitors were conducted by adding 5 µL of a 2 mg/mL enzyme stock solution to an assay mixture with a 1 mL total volume, and taking into account that XYZase is a monomeric enzyme with a molecular mass of 45,000 Daltons, what is the keat of XYZase given in s-¹ (give the answer in two significant figures)? To see a sample kcat* calculation, click on* Hint* in the lower left corner to open the lower panel.* nM x 107s-1
INITIAL INVESTIGATION Run kinetics experiments on XYZase without inhibitors and with compound X. Results: The compound found in the coffee (compound X) has yet to be identified, but using the small amount extracted, you have discovered that it is an inhibitor of XYZase. Given this revelation, it seems clear that this compound is the most likely murder weapon. Shown below is the data you have gathered. Note: [S] is given in nM; other values represent observed Vo values given in units of mol product formed per minute. Vo (no inhibitor) [S] (nM) 50 75 150 400 yes From the limited data you have gathered, does it appear that XYZase follows Michaelis-Menten kinetics? no 0.14 0.19 0.32 0.47 6.3 Vo (Cmpd X) 0.040 0.050 0.080 0.14 What is the Km for XYZase within two significant figures? Incorrect 190 Incorrect Assuming that the experiments performed in the absence of inhibitors were conducted by adding 5 µL of a 2 mg/mL enzyme stock solution to an assay mixture with a 1 mL total volume, and taking into account that XYZase is a monomeric enzyme with a molecular mass of 45,000 Daltons, what is the keat of XYZase given in s-¹ (give the answer in two significant figures)? To see a sample kcat* calculation, click on* Hint* in the lower left corner to open the lower panel.* nM x 107s-1
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
![INITIAL INVESTIGATION
Run kinetics experiments on XYZase without inhibitors and with compound X.
Results: The compound found in the coffee (compound X) has yet to be identified, but using the small amount extracted, you
have discovered that it is an inhibitor of XYZase. Given this revelation, it seems clear that this compound is the most likely
murder weapon. Shown below is the data you have gathered. Note: [S] is given in nM; other values represent observed Vo
values given in units of mol product formed per minute.
Vo (no inhibitor)
[S] (nM)
50
75
150
400
yes
From the limited data you have gathered, does it appear that XYZase follows Michaelis-Menten kinetics?
no
0.14
0.19
0.32
0.47
6.3
Vo (Cmpd X)
0.040
0.050
0.080
0.14
What is the Km for XYZase within two significant figures?
Incorrect
190
Incorrect
Assuming that the experiments performed in the absence of inhibitors were conducted by adding 5 µL of a 2 mg/mL enzyme
stock solution to an assay mixture with a 1 mL total volume, and taking into account that XYZase is a monomeric enzyme with
a molecular mass of 45,000 Daltons, what is the keat of XYZase given in s-¹ (give the answer in two significant figures)?
To see a sample kcat* calculation, click on* Hint* in the lower left corner to open the lower panel.*
nM
x 107s-1](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe3812fa8-aeb2-4a3b-b7d6-495bd586aec9%2F6a748a5c-2a03-4765-9488-94dcdd71a5d4%2Fz6wbtkb_processed.png&w=3840&q=75)
Transcribed Image Text:INITIAL INVESTIGATION
Run kinetics experiments on XYZase without inhibitors and with compound X.
Results: The compound found in the coffee (compound X) has yet to be identified, but using the small amount extracted, you
have discovered that it is an inhibitor of XYZase. Given this revelation, it seems clear that this compound is the most likely
murder weapon. Shown below is the data you have gathered. Note: [S] is given in nM; other values represent observed Vo
values given in units of mol product formed per minute.
Vo (no inhibitor)
[S] (nM)
50
75
150
400
yes
From the limited data you have gathered, does it appear that XYZase follows Michaelis-Menten kinetics?
no
0.14
0.19
0.32
0.47
6.3
Vo (Cmpd X)
0.040
0.050
0.080
0.14
What is the Km for XYZase within two significant figures?
Incorrect
190
Incorrect
Assuming that the experiments performed in the absence of inhibitors were conducted by adding 5 µL of a 2 mg/mL enzyme
stock solution to an assay mixture with a 1 mL total volume, and taking into account that XYZase is a monomeric enzyme with
a molecular mass of 45,000 Daltons, what is the keat of XYZase given in s-¹ (give the answer in two significant figures)?
To see a sample kcat* calculation, click on* Hint* in the lower left corner to open the lower panel.*
nM
x 107s-1
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