3. Hexokinase catalyzes the first reaction in glycolysis and phosphorylates D-glucose to D-gluc 6-phosphate using ATP as the source of the phosphate. Under conditions of pH 7, 25°C and a Hexokinase concentration of 3.0 nmol/mL, the KM for Hexokinase for the substrate glucose was determined to be 3.0 x 10 M. a. When the glucose concentration was set to 160 μM, the initial rate of the reaction was found to be 65.0 μmol/(mL-s). What is Vmax for Hexokinase under these conditions? b. What is the turnover number for Hexokinase under these conditions? c. What is the catalytic efficiency for Hexokinase under these conditions?

3. Hexokinase catalyzes the first reaction in glycolysis and phosphorylates D-glucose to D-gluc 6-phosphate using ATP as the source of the phosphate. Under conditions of pH 7, 25°C and a Hexokinase concentration of 3.0 nmol/mL, the KM for Hexokinase for the substrate glucose was determined to be 3.0 x 10 M. a. When the glucose concentration was set to 160 μM, the initial rate of the reaction was found to be 65.0 μmol/(mL-s). What is Vmax for Hexokinase under these conditions? b. What is the turnover number for Hexokinase under these conditions? c. What is the catalytic efficiency for Hexokinase under these conditions?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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