Acid phosphatase hydrolyses PNPP to pNP. Acid phosphatase has a K, = 0.04 mM. How low would the pNP concentration have to fall to decrease the rate of hydrolysis in the reaction mixture to 75% of V? (Assume that the Michaelis-Menten equation is applicable.) %3D max

Acid phosphatase hydrolyses PNPP to pNP. Acid phosphatase has a K, = 0.04 mM. How low would the pNP concentration have to fall to decrease the rate of hydrolysis in the reaction mixture to 75% of V? (Assume that the Michaelis-Menten equation is applicable.) %3D max

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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Question

Acid phosphatase hydrolyses PNPP to pNP. Acid phosphatase has a K= 0.04 mM.
How low would the pNP concentration have to fall to decrease the rate of hydrolysis in
the reaction mixture to 75% of Vmax? (Assume that the Michaelis-Menten equation is
applicable.)

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