Malate synthase in E. coli catalyzes the reaction Acetyl-CoA + glyoxylate + H₂O malate + CoA-SH + H+ The experimentally measured Km for acetyl-CoA is 9 x 10-6 M. In a growing culture of E. coli, the measured concentration of acetyl-CoA is 6 x 10-4 M. Complete the passage based on these experimental results. Malate synthase is glyoxylate, the reaction rate is limited by with the substrate acetyl-CoA. If [glyoxylate] is below the Km for at Vmax. and malate synthase

Malate synthase in E. coli catalyzes the reaction Acetyl-CoA + glyoxylate + H₂O malate + CoA-SH + H+ The experimentally measured Km for acetyl-CoA is 9 x 10-6 M. In a growing culture of E. coli, the measured concentration of acetyl-CoA is 6 x 10-4 M. Complete the passage based on these experimental results. Malate synthase is glyoxylate, the reaction rate is limited by with the substrate acetyl-CoA. If [glyoxylate] is below the Km for at Vmax. and malate synthase

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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The protein catalase catalyzes the reaction 2H,0,(aq) – 2 H,0O(0) + 0,(g) and has a Michaelis–Menten constant of KM = 25 mM and a turnover number of 4.0 × 107 s-1. The total enzyme concentration is 0.016 µM and the initial substrate concentration is 6.58 µM. Catalase has a single active site. Calculate the value of Rmax (often written as Vmax ) for this enzyme. Rmax mM-s- Calculate the initial rate, R (often written as Vo), of this reaction. R = mM-s-
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