Malate synthase in E. coli catalyzes the reaction Acetyl-CoA + glyoxylate + H₂O malate + CoA-SH + H+ The experimentally measured Km for acetyl-CoA is 9 x 10-6 M. In a growing culture of E. coli, the measured concentration of acetyl-CoA is 6 x 10-4 M. Complete the passage based on these experimental results. Malate synthase is glyoxylate, the reaction rate is limited by with the substrate acetyl-CoA. If [glyoxylate] is below the Km for at Vmax. and malate synthase
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
![Malate synthase in E. coli catalyzes the reaction
Acetyl-CoA + glyoxylate + H₂O
malate + CoA-SH + H+
The experimentally measured Km for acetyl-CoA is 9 x 10-6 M. In a growing culture of E. coli, the measured concentration
of acetyl-CoA is 6 x 10-4 M.
Complete the passage based on these experimental results.
Malate synthase is
glyoxylate, the reaction rate is limited by
with the substrate acetyl-CoA. If [glyoxylate] is below the Km for
at Vmax.
and malate synthase](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F664a912e-25f8-4c69-9b55-94f0ad421fac%2F18a74b23-d5b4-4d2d-8d35-07345753b9c9%2Ftcj4bbg_processed.jpeg&w=3840&q=75)
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