1. Determining Km and Vmax. You have recently discovered a new enzyme homeworkase which destroys your homework assignments. In order to characterize this enzyme, you wish to determine the Km and Vmax To do so, you make up several different concentrations of substrate (keeping the enzyme concentration constant) and run the reaction. You obtain the following results. [Substrate] (pages/liter) reaction rate (pages/liter/min) 65 63 51 1.0 x 10³ 5.0 x 104 1.0 x 10-4 5.0 x 105 3.0 x 10-6 2.0 x 105 1.0 x 105 5.0 x 10€ 1.0 x 10€ 5.0 x 10-7 42 33 27 17 9.5 2.2 1.1 (a) Is this data consistent with Michaelis Menten kinetics? (HINT: plot the data). Do not forget to include your well-labeled graph/plot! (b) Determine the Vmax and Km for this enzyme using (i) Lineweaver-Burk and an (ii) Eadie-Hofstee plot (you will have to look up the latter). How do the results compare with each other? Do not forget to include your well-labeled plots!
1. Determining Km and Vmax. You have recently discovered a new enzyme homeworkase which destroys your homework assignments. In order to characterize this enzyme, you wish to determine the Km and Vmax To do so, you make up several different concentrations of substrate (keeping the enzyme concentration constant) and run the reaction. You obtain the following results. [Substrate] (pages/liter) reaction rate (pages/liter/min) 65 63 51 1.0 x 10³ 5.0 x 104 1.0 x 10-4 5.0 x 105 3.0 x 10-6 2.0 x 105 1.0 x 105 5.0 x 10€ 1.0 x 10€ 5.0 x 10-7 42 33 27 17 9.5 2.2 1.1 (a) Is this data consistent with Michaelis Menten kinetics? (HINT: plot the data). Do not forget to include your well-labeled graph/plot! (b) Determine the Vmax and Km for this enzyme using (i) Lineweaver-Burk and an (ii) Eadie-Hofstee plot (you will have to look up the latter). How do the results compare with each other? Do not forget to include your well-labeled plots!
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Concept explainers
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
Question
![1. Determining Km and Vmax- You have recently discovered a new enzyme homeworkase which destroys
your homework assignments. In order to characterize this enzyme, you wish to determine the Km and Vmax.
To do so, you make up several different concentrations of substrate (keeping the enzyme concentration
constant) and run the reaction. You obtain the following results.
[Substrate] (pages/liter)
reaction rate (pages/liter/min)
65
1.0 x 10-³
5.0 x 10-4
1.0 x 104
5.0 x 10.5
3.0 x 10-5
2.0 x 10-5
1.0 x 10-5
5.0 x 10€
1.0 x 10€
5.0 x 10-7
63
51
42
33
27
17
9.5
2.2
1.1
(a) Is this data consistent with Michaelis Menten kinetics? (HINT: plot the data). Do not forget to include
your well-labeled graph/plot!
(b) Determine the Vmax and Km for this enzyme using (i) Lineweaver-Burk and an (ii) Eadie-Hofstee plot
(you will have to look up the latter). How do the results compare with each other? Do not forget to
include your well-labeled plots!](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F16ce2476-f3b7-4581-8eb1-155e620ff9a7%2F1623d7fe-6f85-4ca5-aa57-5502c59fd70b%2F2p0ngx4_processed.png&w=3840&q=75)
Transcribed Image Text:1. Determining Km and Vmax- You have recently discovered a new enzyme homeworkase which destroys
your homework assignments. In order to characterize this enzyme, you wish to determine the Km and Vmax.
To do so, you make up several different concentrations of substrate (keeping the enzyme concentration
constant) and run the reaction. You obtain the following results.
[Substrate] (pages/liter)
reaction rate (pages/liter/min)
65
1.0 x 10-³
5.0 x 10-4
1.0 x 104
5.0 x 10.5
3.0 x 10-5
2.0 x 10-5
1.0 x 10-5
5.0 x 10€
1.0 x 10€
5.0 x 10-7
63
51
42
33
27
17
9.5
2.2
1.1
(a) Is this data consistent with Michaelis Menten kinetics? (HINT: plot the data). Do not forget to include
your well-labeled graph/plot!
(b) Determine the Vmax and Km for this enzyme using (i) Lineweaver-Burk and an (ii) Eadie-Hofstee plot
(you will have to look up the latter). How do the results compare with each other? Do not forget to
include your well-labeled plots!
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