During a Michaelis Menten Kinetics experiment the following the standard curve and absorbance of one of the 5 min enzymatic reaction was obtained. • Calculate the initial velocity for the enzymatic reaction • Write a title for the standard curve and comment on the correlation between the standard curve data. 1.200 1.000 0.800 0.600 0.400 0.200 0.000 - Absorbance of enzymatic product (p-nitrophenol) y= 0.837x + 0.1182 R=0.9791 0.000 1.000 2.000 p nitropheol concentration absorbance
During a Michaelis Menten Kinetics experiment the following the standard curve and absorbance of one of the 5 min enzymatic reaction was obtained. • Calculate the initial velocity for the enzymatic reaction • Write a title for the standard curve and comment on the correlation between the standard curve data. 1.200 1.000 0.800 0.600 0.400 0.200 0.000 - Absorbance of enzymatic product (p-nitrophenol) y= 0.837x + 0.1182 R=0.9791 0.000 1.000 2.000 p nitropheol concentration absorbance
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:During a Michaelis Menten Kinetics experiment the following the standard curve
and absorbance of one of the 5 min enzymatic reaction was obtained.
• Calculate the initial velocity for the enzymatic reaction
• Write a title for the standard curve and comment on the correlation
between the standard curve data.
1.200
1.000
0.800
0.600
0.400
0.200
0.000
Absorbance of enzymatic
product (D-nitrophenol)
y= 0.837x +0.1182
R2 =0.9791
0.000
1.000
2.000
p nitropheol concentration
absorbance
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