5. Consider a standard C-18 reversed-phase column. Suppose you applied a globular protein to the column and measured the elution time. You then ran the column a second time using the same protein, except you heat-denatured the protein before applying it to the column. Assume that both the native and denatured proteins are soluble, and that they do not change their respective conformations during the column run. Which form should migrate through the column faster, the native form of the protein, or the denatured form of the protein? Explain briefly.

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5. Consider a standard C-18 reversed-phase column. Suppose you applied a globular
protein to the column and measured the elution time. You then ran the column a
second time using the same protein, except you heat-denatured the protein before
applying it to the column. Assume that both the native and denatured proteins are
soluble, and that they do not change their respective conformations during the column
run. Which form should migrate through the column faster, the native form of the
protein, or the denatured form of the protein? Explain briefly.
Transcribed Image Text:5. Consider a standard C-18 reversed-phase column. Suppose you applied a globular protein to the column and measured the elution time. You then ran the column a second time using the same protein, except you heat-denatured the protein before applying it to the column. Assume that both the native and denatured proteins are soluble, and that they do not change their respective conformations during the column run. Which form should migrate through the column faster, the native form of the protein, or the denatured form of the protein? Explain briefly.
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