5. Consider a standard C-18 reversed-phase column. Suppose you applied a globular protein to the column and measured the elution time. You then ran the column a second time using the same protein, except you heat-denatured the protein before applying it to the column. Assume that both the native and denatured proteins are soluble, and that they do not change their respective conformations during the column run. Which form should migrate through the column faster, the native form of the protein, or the denatured form of the protein? Explain briefly.
5. Consider a standard C-18 reversed-phase column. Suppose you applied a globular protein to the column and measured the elution time. You then ran the column a second time using the same protein, except you heat-denatured the protein before applying it to the column. Assume that both the native and denatured proteins are soluble, and that they do not change their respective conformations during the column run. Which form should migrate through the column faster, the native form of the protein, or the denatured form of the protein? Explain briefly.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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