5. Consider a standard C-18 reversed-phase column. Suppose you applied a globularprotein to the column and measured the elution time. You then ran the column asecond time using the same protein, except you heat-denatured the protein beforeapplying it to the column. Assume that both the native and denatured proteins aresoluble, and that they do not change their respective conformations during the columnrun. Which form should migrate through the column faster, the native form of theprotein, or the denatured form of the protein? Explain briefly.

In a standard C-18 reversed-phase column, the stationary phase is nonpolar (hydrophobic) and the…

Answered: 5. Consider a standard C-18…

Chemistry

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ISBN:9781305957404

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Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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What happens when a mixture of positively-charged Protein A and negatively-charged Protein B is loaded onto an anion-exchange column? Both proteins bind to the resin. Neither protein binds to the resin. Protein A binds to the resin and Protein B remains unbound. Protein B binds to the resin and Protein A remains unbound
Of the following methods listed below, which could not be used to obtain a reasonable estimate of the molecular mass of a monomeric protein? Select all that apply. O Electrospray ionization mass spectrometrylsoelectric focusing O Gel filtration O All of these methods could be used O Isoelectric focusing O Sodium dodecyl sulfate polyacrylamide gel electrophoresis O Total amino acid sequence determination by Edman degradation
Pls solve this question correctly instantly in 5 min i will give u 3 like for sure
identifying specific interactions between residues in a protein www-awu.aleks.com 00 Imagine the main chain of a protein bends back on itself, so that two amino acid residues R, and R, come close to each other. Here's what R looks like: CH- CH₂ CH₂ 0=0 NH₂ O yes X Could R, form a disulfide bond with R₂ at physiological pH, if R2 were the right kind of residue? O no If you said R, could form a disulfide bond with R₂, what would R₂ have to be? If there's only one possibility, write its 3-letter code. If there is more than one possibility, write the 3-letter code of any of them. Explanation Check PH LLC. All Rights Reserved Terms of Use Privacy Center 1 Accense
Chemistry please cleary and step by step explain this question. also if possible type the explanation and answer. thank you.
Consider a "tripeptide" with the following features (at pH = 10.8) • The N-terminal amino acid is leucine • The middle amino acid is aspartate • The C-terminal amino acid is lysine i. What is the 1-letter symbol for each of these amino acids il. Calculate the net charge of the predominant ionic state of this tripeptide at pH 10.8. Show work.
ONLY ANSWER A AND B
A mixture of three proteins with pl = 7.5, 6.5, and 5.5 is separated using a DEAE-Sepharose column by eluting (washing) with buffer and NaCl. The column is eluted with aqueous buffer with a pH of 8.0, and an increasing [NaCl]. Which of the following proteins will elute from the column last? A The protein with pl = 5.5 B The protein with pl = 6.5 ☑ The protein with pl = 7.5 D These three proteins will not be separated, and will elute together. Your answer
The isoelectric point (pl) can best be calculated by: Averaging the sums of all pkas. Averaging the sum of the two pKas closest to physiological pH. Averaging the two pKas "sandwiching" the zwitterionic form. Averaging the two pKas of the alpha-amino and alpha-carboxy groups.
what is The method that would be BEST to use to monitor protein secondary structure during the titration of a denaturing agent?
Can someone check my answers and see if they are correct?
1 Submit Q10. !! Unanswered Q10. An average amino acid is about 110 Da (Daltons). If there are 1273 amino acids in the SARS-CoV-2 spike protein, what do you expect the mass of spike to be? How does that compare to the expected mass of about 200 KDa? more than expected by about 90 Da Less than expected by about 90 Da B. more than expected by by about 60 KDa less than expected by about 60 KDa Submit odf 0/11 answered

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