A mixture of three proteins with pl = 7.5, 6.5, and 5.5 is separated using a DEAE-Sepharose column by eluting (washing) with buffer and NaCl. The column is eluted with aqueous buffer with a pH of 8.0, and an increasing [NaCl]. Which of the following proteins will elute from the column last? A The protein with pl = 5.5 B The protein with pl = 6.5 ☑ The protein with pl = 7.5 D These three proteins will not be separated, and will elute together. Your answer

A mixture of three proteins with pl = 7.5, 6.5, and 5.5 is separated using a DEAE-Sepharose column by eluting (washing) with buffer and NaCl. The column is eluted with aqueous buffer with a pH of 8.0, and an increasing [NaCl]. Which of the following proteins will elute from the column last? A The protein with pl = 5.5 B The protein with pl = 6.5 ☑ The protein with pl = 7.5 D These three proteins will not be separated, and will elute together. Your answer

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

A mixture of three proteins with pl = 7.5, 6.5, and 5.5 is separated using a
DEAE-Sepharose column by eluting (washing) with buffer and NaCl. The
column is eluted with aqueous buffer with a pH of 8.0, and an increasing
[NaCl]. Which of the following proteins will elute from the column last?
A The protein with pl = 5.5
B
The protein with pl = 6.5
☑
The protein with pl = 7.5
D
These three proteins will not be separated, and will elute
together.
Your answer

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