0.9The enzyme from the previous problem was further purified from the supernatant, after which a (Bradford) protein assay using coomassie bluewas performed. The following standard curve was established using known concentrations of the enzyme.5950.80.70.60.580.40.30.20.1y=0.5617x+0.0119R²=0.987800.5Protein Concentration (mg/mL)A. At what wavelength is the sample measured at?1.5B. Write out the equation relating OD595 and protein concentration.C. After purification, the protein sample was diluted 10-fold and then reacted with Coomassie Blue that resulted in an OD595 of 0.7. What wasthe concentration of protein in the supernatant?D. The purified protein was concentrated to a final volume of 1 L. What was the final amount of purified protein recovered?200 g of the hulls initially at 25°C was placed into a perfectly insulated calorimeter with 500 g of isopropyl alcohol (IPA) at 5°C without stirring.At equilibrium, the temperature of the IPA was found to be 13.7°C.=A. Ignoring heat contributions from the calorimeter itself, calculate the specific heat capacity of the almond hulls. (Cp.IPA 2.68 kJ/kg/K).B. If the calorimeter container, initially at 25°C, had contributed 1.2 kJ of heat in the process, what is the actual specific heat of the almondhulls?A peeled cucumber is vacuum cooled. The amount of moisture removed was 2.3 g and the temperature change of the cucumber was 15° C. Howmuch heat was removed from the cucumber?

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The enzyme from the previous problem was further purified from the supernatant, after which a (Bradford) protein assay using coomassie blue was performed. The following standard curve was established using known concentrations of the enzyme. 0.9 0.8 0.7 0.6 0.5 804 0.3 0.2 y=0.5617x+0.0119 R2=0.9878 0.1 0 0 0.5 1 1.5 Protein Concentration (mg/mL) A. At what wavelength is the sample measured at? B. Write out the equation relating OD595 and protein concentration. 4 C. After purification, the protein sample was diluted 10-fold and then reacted with Coomassie Blue that resulted in an OD595 of 0.7. What was the concentration of protein in the supernatant?

The enzyme from the previous problem was further purified from the supernatant, after which a (Bradford) protein assay using coomassie blue was performed. The following standard curve was established using known concentrations of the enzyme. 0.9 0.8 0.7 0.6 0.5 804 0.3 0.2 y=0.5617x+0.0119 R2=0.9878 0.1 0 0 0.5 1 1.5 Protein Concentration (mg/mL) A. At what wavelength is the sample measured at? B. Write out the equation relating OD595 and protein concentration. 4 C. After purification, the protein sample was diluted 10-fold and then reacted with Coomassie Blue that resulted in an OD595 of 0.7. What was the concentration of protein in the supernatant?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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