Enz-SH + Ag* –→ Enz-S-Ag + H* The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. To 10.0 mL of a solution containing 3.5 mg/mL of a pure enzyme, an investigator added just enough AGNO, to completely inactivate the enzyme. A total of 0.536 umol AGNO, was required. Calculate the minimum molecular weight (M,) of the enzyme. minimum M, : The determined M, is not exact, but it is a minimum value for the M, of the enzyme. Why does the M, value obtained this way give only the minimum molecular weight? There is only one polypeptide chain. There are no disulfide bonds in the protein. There is only one titratable -SH group per protein molecule. O The Ag+ would also react with the terminal carboxyl group.

Enz-SH + Ag* –→ Enz-S-Ag + H* The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. To 10.0 mL of a solution containing 3.5 mg/mL of a pure enzyme, an investigator added just enough AGNO, to completely inactivate the enzyme. A total of 0.536 umol AGNO, was required. Calculate the minimum molecular weight (M,) of the enzyme. minimum M, : The determined M, is not exact, but it is a minimum value for the M, of the enzyme. Why does the M, value obtained this way give only the minimum molecular weight? There is only one polypeptide chain. There are no disulfide bonds in the protein. There is only one titratable -SH group per protein molecule. O The Ag+ would also react with the terminal carboxyl group.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

Enz-SH + Ag* -
Enz-S-Ag + H*
The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. To
10.0 mL of a solution containing 3.5 mg/mL of a pure enzyme, an investigator added just enough AGNO, to completely
inactivate the enzyme. A total of 0.536 umol AGNO, was required. Calculate the minimum molecular weight (M,) of
the enzyme.
minimum M, =
The determined M, is not exact, but it is a minimum value for the M, of the enzyme.
Why does the M, value obtained this way give only the minimum molecular weight?
There is only one polypeptide chain.
There are no disulfide bonds in the protein.
There is only one titratable -SH group per protein molecule.
The Ag* would also react with the terminal carboxyl group.

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Question 1 of 10 Many enzymes are irreversibly inhibited by heavy metal ions such as Hg*. Cut, or Ag, which can react with essential sulfhydryl groups to form mercaptides. Enz-SH + Ag* Enz-S-Ag + H* The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. An investigator added just enough AgNO, to completely inactivate a 10.0 mL solution containing 1.0 mg/mL enzyme. A total of 0.342 umol of AgNO, was required. Calculate the minimum molecular weight of the enzyme. g/mol enzyme minimum molecular weight: Why does the value obtained in this way give only the minimum molecular weight?