1. Suppose an enzyme only shows activity when the side chain -COOH group of a glutamic acid in the active site is protonated. It is known that the pKa of the side chain -COOH group is 4.07. a) Is the enzyme more active at pH 2.4 or 5.7? Explain. b) What percentage of the enzymes is active at pH = 6.5? Explain. c) At what pH will the enzyme show 78% of maximal activity?

Chemistry for Today: General, Organic, and Biochemistry
9th Edition
ISBN:9781305960060
Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Chapter20: Enzymes
Section: Chapter Questions
Problem 20.51E
icon
Related questions
Question

I realllyyy need help with this one please help 

1. Suppose an enzyme only shows activity when the side chain -COOH group of a glutamic acid
in the active site is protonated. It is known that the pKa of the side chain -COOH group is 4.07.
a) Is the enzyme more active at pH 2.4 or 5.7? Explain.
b) What percentage of the enzymes is active at pH = 6.5? Explain.
c) At what pH will the enzyme show 78% of maximal activity?
Transcribed Image Text:1. Suppose an enzyme only shows activity when the side chain -COOH group of a glutamic acid in the active site is protonated. It is known that the pKa of the side chain -COOH group is 4.07. a) Is the enzyme more active at pH 2.4 or 5.7? Explain. b) What percentage of the enzymes is active at pH = 6.5? Explain. c) At what pH will the enzyme show 78% of maximal activity?
Expert Solution
steps

Step by step

Solved in 4 steps

Blurred answer
Knowledge Booster
Spectroanalytical Methods
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Chemistry for Today: General, Organic, and Bioche…
Chemistry for Today: General, Organic, and Bioche…
Chemistry
ISBN:
9781305960060
Author:
Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:
Cengage Learning
Introduction to General, Organic and Biochemistry
Introduction to General, Organic and Biochemistry
Chemistry
ISBN:
9781285869759
Author:
Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:
Cengage Learning
Introductory Chemistry: An Active Learning Approa…
Introductory Chemistry: An Active Learning Approa…
Chemistry
ISBN:
9781305079250
Author:
Mark S. Cracolice, Ed Peters
Publisher:
Cengage Learning
General, Organic, and Biological Chemistry
General, Organic, and Biological Chemistry
Chemistry
ISBN:
9781285853918
Author:
H. Stephen Stoker
Publisher:
Cengage Learning
Organic And Biological Chemistry
Organic And Biological Chemistry
Chemistry
ISBN:
9781305081079
Author:
STOKER, H. Stephen (howard Stephen)
Publisher:
Cengage Learning,
Pushing Electrons
Pushing Electrons
Chemistry
ISBN:
9781133951889
Author:
Weeks, Daniel P.
Publisher:
Cengage Learning