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Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter18: Carboxylic Acids

Section: Chapter Questions

Problem 18.54P

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Could someone explain what this is saying? I would like a better understanding. FIGURE D

CPP32 (Caspase-3) protease activity. Aliquots of cell lysates (50
μL) were incubated with an equal volume (50 μL) of the reaction
buffer and 5 μL of 7-amino-4-trifluoromethyl coumarin (AFCDEVD) for 1 hour at 37˚C (Clontech). The shift in fluorescence
emission of AFC-DEVD, on its proteolysis to free AFC by the
protease, was detected in a fluorometer, using a 400 nm excitation
filter and 505 nm emission filter.

a
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Sodium ascorbate (mM)
Sodium ascorbate (mM)
d
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Sodium ascorbate (mM)
Sodium ascorbate (mM)
Bax (% of control)
p21 (% of control)
*
Caspase-3 (% of control)
p53 (% of control)

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