The isoelectric point, pl, of the protein horse liver alcohol dehydrogenase is 6.8, while that of beta-glucuronidase is 5.1. What is the net charge of horse liver alcohol dehydrogenase at pH 5.1 ?[ What is the net charge of beta-glucuronidase at pH 4.5 ?[ The isoelectric point of threonine is 5.6 ; lysine, 9.74. During paper electrophoresis at pH 4.5, toward which electrode does threonine migrate? [ During paper electrophoresis at pH 7.3, toward which electrode does lysine migrate? [
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
In the provided information and questions, we deal with the concept of isoelectric points (pI) and net charges of proteins and amino acids at specific pH levels. Understanding the behavior of biomolecules like proteins and amino acids in relation to pH is essential in various fields of biochemistry and molecular biology. In this introduction, I will provide an overview of the concept of pI and its significance, as well as an explanation of how the net charge of biomolecules changes with respect to pH.
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