Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter20: Carbohydrates
Section: Chapter Questions
Problem 20.66P
Related questions
Question
An enzymatic assay was performed using the enzyme phophofructokinase-1, which catalyses the committed step of the glycolysis pathway. What is the final concentration of the fructose 6-phosphate substrate in a test tube containing the following: 250 µL of 150pM fructose 6-phophate, 100 µL 20 mg.ml-1 phosphofructoskinase-1 and 150 µL 100mM buffer pH 7.5
Transcribed Image Text:DO POCHz
H
Н
НО
ОН
ОН Н
CH₂OH
31
Do3POCHz
ATP ADP
и
Phosphofructokinase-1
Fructose 6-phosphate
Unnumbered figure pg 333 Principles of Biochemistry, 4/e
©2006 Pearson Prentice Hall, Inc.
Н
О
Н Но
ОН
CH₂OPO
3 1
ОН Н
Fructose 1,6-bisphosphate
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