Question 1 of 10 Many enzymes are irreversibly inhibited by heavy metal ions such as Hg. Cu, or Ag*, which can react with essential sulfhydryl groups to form mercaptides. Enz-SH + Ag Enz-S-Ag + H* - The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. An investigator added just enough AgNO, to completely inactivate a 10.0 mL solution containing 1.0 mg/ml. enzyme. A total of 0.342 umol of AgNO, was required. Calculate the minimum molecular weight of the enzyme. enzyme minimum molecular weight: g/mol Why does the value obtained in this way give only the minimum molecular weight?

Question 1 of 10 Many enzymes are irreversibly inhibited by heavy metal ions such as Hg. Cu, or Ag, which can react with essential sulfhydryl groups to form mercaptides. Enz-SH + Ag Enz-S-Ag + H - The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. An investigator added just enough AgNO, to completely inactivate a 10.0 mL solution containing 1.0 mg/ml. enzyme. A total of 0.342 umol of AgNO, was required. Calculate the minimum molecular weight of the enzyme. enzyme minimum molecular weight: g/mol Why does the value obtained in this way give only the minimum molecular weight?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 14.15QAP

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Enz-SH + Ag* - Enz-S-Ag + H* The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. To 10.0 mL of a solution containing 3.5 mg/mL of a pure enzyme, an investigator added just enough AGNO, to completely inactivate the enzyme. A total of 0.536 umol AGNO, was required. Calculate the minimum molecular weight (M,) of the enzyme. minimum M, = The determined M, is not exact, but it is a minimum value for the M, of the enzyme. Why does the M, value obtained this way give only the minimum molecular weight? There is only one polypeptide chain. There are no disulfide bonds in the protein. There is only one titratable -SH group per protein molecule. The Ag* would also react with the terminal carboxyl group.
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