Question 1 of 10 Many enzymes are irreversibly inhibited by heavy metal ions such as Hg. Cu, or Ag*, which can react with essential sulfhydryl groups to form mercaptides. Enz-SH + Ag Enz-S-Ag + H* - The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. An investigator added just enough AgNO, to completely inactivate a 10.0 mL solution containing 1.0 mg/ml. enzyme. A total of 0.342 umol of AgNO, was required. Calculate the minimum molecular weight of the enzyme. enzyme minimum molecular weight: g/mol Why does the value obtained in this way give only the minimum molecular weight?

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Question 1 of 10
Many enzymes are irreversibly inhibited by heavy metal ions such as Hg*. Cut, or Ag, which can react with essential
sulfhydryl groups to form mercaptides.
Enz-SH + Ag*
Enz-S-Ag + H*
The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively.
An investigator added just enough AgNO, to completely inactivate a 10.0 mL solution containing 1.0 mg/mL enzyme. A total of
0.342 umol of AgNO, was required.
Calculate the minimum molecular weight of the enzyme.
g/mol
enzyme minimum molecular weight:
Why does the value obtained in this way give only the minimum molecular weight?
Transcribed Image Text:Question 1 of 10 Many enzymes are irreversibly inhibited by heavy metal ions such as Hg*. Cut, or Ag, which can react with essential sulfhydryl groups to form mercaptides. Enz-SH + Ag* Enz-S-Ag + H* The affinity of Ag* for sulfhydryl groups is so great that Ag* can be used to titrate -SH groups quantitatively. An investigator added just enough AgNO, to completely inactivate a 10.0 mL solution containing 1.0 mg/mL enzyme. A total of 0.342 umol of AgNO, was required. Calculate the minimum molecular weight of the enzyme. g/mol enzyme minimum molecular weight: Why does the value obtained in this way give only the minimum molecular weight?
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